Abstract: Simulation models are frequently applied to determine the production potential of forage grasses under various scenarios, including climate change. Thorough calibrations and evaluations of forage grass models can help improve their applicability. This study evaluated the ability of the Light Interception and Utilization Simulator-GRAss (LINGRA) model to predict biomass yield of timothy (Phleum pratense L. cv. Grindstad) in the Nordic countries. Variety trial data for the first and second year after establishment were obtained for seven locations: Jokioinen, Finland (60 degrees 48 ‘ N; 23 degrees 29 ‘ E), Maaninka, Finland (63 degrees 09 ‘ N; 27 degrees 18 ‘ E), Korpa, Iceland (64 degrees 09 ‘ N; 21 degrees 45 ‘ W), Srheim, Norway (58 degrees 41 ‘ N; 5 degrees 39 ‘ E), Lillerud, Sweden (59 degrees 24’ N; 13 degrees 16 ‘ E), Ostersund, Sweden (63 degrees 15 ‘ N; 14 degrees 34 ‘ E) and Ulna Sweden (63 degrees 49 ‘ N; 20 degrees 13 ‘ E) from 1992 to 2012. Two calibrations of the LINGRA model were carried out using Bayesian techniques. In the first of these (SRrheim calibration), data on biomass yield and underlying variables obtained from independent field trials at Srheim were used. In the second (Nordic calibration), biomass data from the other locations were used as well. The model was validated against the remaining set of biomass yields from all locations not included in the Nordic calibration. The observed total seasonal yield the first and second year after establishment was 913 and 991 g DM m(-2) respectively on average across the locations. The corresponding average simulated yield after the Srheim calibration was 1044 (root mean square error (RMSE) 258) and 1112 g DM m(-2) (RMSE 312), respectively. After the Nordic calibration, the simulated average total seasonal yield was 863 (RMSE 242) the first year and 927 g DM m(-2) (RMSE 271) the second year after establishment. The differences between the observed and simulated first cut yield followed the same patterns, whereas the prediction accuracy for second cut yield did not differ substantially between the calibration approaches.Using the parameter set from the Nordic region decreased the model predictability at Srheim compared with only using model parameters derived from this location. These results show that using biomass data from several locations, instead of only one specific location, in the calibration of the LINGRA model improved the overall prediction accuracy of first cut dry matter yield and total seasonal dry matter yield across an environmentally heterogeneous region. To further analyse the usefulness of including multi-site data in forage grass model calibrations, other forage grass models could be evaluated against the same dataset.

Abstract: Agricultural systems models are complex and tend to be over-parameterized with respect to observational datasets. Practical identifiability analysis based on local sensitivity analysis has proved effective in investigating identifiable parameter sets in environmental models, but has not been applied to agricultural systems models. Here, we demonstrate that identifiability analysis improves experimental design to ensure independent parameter estimation for yield and quality outputs of a complex grassland model. The Pasture Simulation model (PaSim) was used to demonstrate the effectiveness of practical identifiability analysis in designing experiments and measurement protocols within phe-notyping experiments with perennial ryegrass. Virtual experiments were designed combining three factors: frequency of measurements, duration of the experiment. and location of trials. Our results demonstrate that (i) PaSim provides sufficient detail in terms of simulating biomass yield and quality of perennial ryegrass for use in breeding, (ii) typical breeding trials are insufficient to parameterize all influential parameters, (iii) the frequency of measurements is more important than the number of growing seasons to improve the identifiability of PaSim parameters, and (iv) identifiability analysis provides a sound approach for optimizing the design of multi-location trials. Practical identifiability analysis can play an important role in ensuring proper exploitation of phenotypic data and cost-effective multi-location experimental designs. Considering the growing importance of simulation models, this study supports the design of experiments and measurement protocols in the phenotyping networks that have recently been organized.

Abstract: Grasslands play important roles in agricultural production and provide a range of ecosystem services. Modelling can be a valuable adjunct to experimental research in order to improve the knowledge and assess the impact of management practices in grassland systems. In this study, the PaSim model was assessed for its ability to simulate plant biomass production, soil temperature, water content, and total and heterotrophic soil respiration in Mediterranean grasslands. The study site was the extensively managed sheep grazing system at the Berchidda‐Monti Observatory (Sardinia, Italy), from which two data sets were derived for model calibration and validation respectively. A new model parameterization was derived for Mediterranean conditions from a set of eco‐physiological parameters. With the exception of heterotrophic respiration (Rh), for which modelling efficiency (EF) values were negative, the model outputs were in agreement with observations (e.g., EF ranging from ~0.2 for total soil respiration to ~0.7 for soil temperature). These results support the effectiveness of PaSim to simulate C cycle components in Mediterranean grasslands. The study also highlights the need of further model development to provide better representation of the seasonal dynamics of Mediterranean annual species‐rich grasslands and associated peculiar Rh features, for which the modelling is only implicitly being undertaken by the current PaSim release.