Abstract: Impact of climate change on crop growth, groundwater recharge and nitrogen leaching in winter wheat production in Germany was assessed using the agro-ecosystem model HERMES with a downscaled (WETTREG) climate change scenario A1B from the ECHAM5 global circulation model. Three alternative algorithms describing the impact of atmospheric CO2 concentration on crop growth (a simple Farquhar-type algorithm, a combined light-use efficiency – maximum assimilation approach and a simple scaling of the maximum assimilation rate) in combination with a Penman-Monteith approach which includes a simple stomata conduction model for evapotranspiration under changing CO2 concentrations were compared within the framework of the HERMES model. The effect of differences in regional climate change, site conditions and different CO2 algorithms on winter wheat yield, groundwater recharge and nitrogen leaching was assessed in 22 regional simulation case studies across Germany. Results indicate that the effects of climate change on wheat production will vary across Germany due to different regional expressions of climate change projection. Predicted yield changes between the reference period (1961-1990) and a future period (2021-2050) range from -0.4 t ha(-1), -0.8 t ha(-1) and -0.6 t ha(-1) at sites in southern Germany to +0.8 t ha(-1), +0.6 t ha(-1) and +0.8 t ha(-1) at coastal regions for the three CO2 algorithms, respectively. On average across all regions, a relative yield change of +0.9%, +3.0%, and +6.0%, respectively, was predicted for Germany. In contrast, a decrease of -11.6% was predicted without the consideration of a CO2 effect. However, simulated yield changes differed even within regions as site conditions had a strong influence on crop growth. Particularly, groundwater-affected sites showed a lower vulnerability to increasing drought risk. Groundwater recharge was estimated to change correspondingly to changes in precipitation. The consideration of the CO2 effect on transpiration in the model led to a prediction of higher rates of annual deep percolation (+16 mm on average across all sites), which was due to higher water-use efficiency of the crops. In contrast to groundwater recharge, simulated nitrogen leaching varied with the choice of the photosynthesis algorithm, predicting a slight reduction in most of the areas. The results underline the necessity of high-resolution data for model-based regional climate change impact assessment and development of adaptation measures. (C) 2013 Elsevier B.V. All rights reserved.