Abstract: Using the detailed field experiment data from 1981 to 2009 at four representative agro-meteorological experiment stations in China, along with the Agricultural Production System Simulator (APSIM) rice-wheat model, we evaluated the impact of sowing/transplanting date on phenology and yield of rice-wheat rotation system (RWRS). We also disentangled the contributions of climate change, modern cultivars, sowing/transplanting density and fertilization management, as well as changes in each climate variables, to yield change in RWRS, in the past three decades. We found that change in sowing/transplanting date did not significantly affect rice and wheat yield in RWRS, although alleviated the negative impact of climate change to some extent. From 1981 to 2009, climate change jointly caused rice and wheat yield change by -17.4 to 1.5 %, of which increase in temperature reduced yield by 0.0-5.8 % and decrease in solar radiation reduced it by 1.5-8.7 %. Cultivars renewal, modern sowing/transplanting density and fertilization management contributed to yield change by 14.4-27.2, -4.7- -0.1 and 2.3-22.2 %, respectively. Our findings highlight that modern cultivars and agronomic management compensated the negative impacts of climate change and played key roles in yield increase in the past three decades.

Abstract: Reactive nitrogen (Nr) is an indispensable nutrient for agricultural production and human alimentation. Simultaneously, agriculture is the largest contributor to Nr pollution, causing severe damages to human health and ecosystem services. The trade-off between food availability and Nr pollution can be attenuated by several key mitigation options, including Nr efficiency improvements in crop and animal production systems, food waste reduction in households and lower consumption of Nr-intensive animal products. However, their quantitative mitigation potential remains unclear, especially under the added pressure of population growth and changes in food consumption. Here we show by model simulations, that under baseline conditions, Nr pollution in 2050 can be expected to rise to 102-156% of the 2010 value. Only under ambitious mitigation, does pollution possibly decrease to 36-76% of the 2010 value. Air, water and atmospheric Nr pollution go far beyond critical environmental thresholds without mitigation actions. Even under ambitious mitigation, the risk remains that thresholds are exceeded.

Abstract: Agricultural nitrous oxide (N2O) pollution resulting from the use of synthetic fertilizers represents a significant contribution to anthropogenic greenhouse gas emissions, providing a rationale for reduced use of nitrogen (N) fertilizers. Nitrogen limitation results in extensive systems rebalancing that remodels metabolism and defence processes. To analyse the regulation underpinning these responses, barley (Horedeum vulgare) seedlings were grown for 7 d under N-deficient conditions until net photosynthesis was 50% lower than in N-replete controls. Although shoot growth was decreased there was no evidence for the induction of oxidative stress despite lower total concentrations of N-containing antioxidants. Nitrogen-deficient barley leaves were rich in amino acids, sugars and tricarboxylic acid cycle intermediates. In contrast to N-replete leaves one-day-old nymphs of the green peach aphid (Myzus persicae) failed to reach adulthood when transferred to N-deficient barley leaves. Transcripts encoding cell, sugar and nutrient signalling, protein degradation and secondary metabolism were over-represented in N-deficient leaves while those associated with hormone metabolism were similar under both nutrient regimes with the exception of mRNAs encoding proteins involved in auxin metabolism and responses. Significant similarities were observed between the N-limited barley leaf transcriptome and that of aphid-infested Arabidopsis leaves. These findings not only highlight significant similarities between biotic and abiotic stress signalling cascades but also identify potential targets for increasing aphid resistance with implications for the development of sustainable agriculture.