Abstract: Agricultural nitrous oxide (N2O) pollution resulting from the use of synthetic fertilizers represents a significant contribution to anthropogenic greenhouse gas emissions, providing a rationale for reduced use of nitrogen (N) fertilizers. Nitrogen limitation results in extensive systems rebalancing that remodels metabolism and defence processes. To analyse the regulation underpinning these responses, barley (Horedeum vulgare) seedlings were grown for 7 d under N-deficient conditions until net photosynthesis was 50% lower than in N-replete controls. Although shoot growth was decreased there was no evidence for the induction of oxidative stress despite lower total concentrations of N-containing antioxidants. Nitrogen-deficient barley leaves were rich in amino acids, sugars and tricarboxylic acid cycle intermediates. In contrast to N-replete leaves one-day-old nymphs of the green peach aphid (Myzus persicae) failed to reach adulthood when transferred to N-deficient barley leaves. Transcripts encoding cell, sugar and nutrient signalling, protein degradation and secondary metabolism were over-represented in N-deficient leaves while those associated with hormone metabolism were similar under both nutrient regimes with the exception of mRNAs encoding proteins involved in auxin metabolism and responses. Significant similarities were observed between the N-limited barley leaf transcriptome and that of aphid-infested Arabidopsis leaves. These findings not only highlight significant similarities between biotic and abiotic stress signalling cascades but also identify potential targets for increasing aphid resistance with implications for the development of sustainable agriculture.

Abstract: Maize (Zea mays L) is one of the most important food crops and very common in all parts of sub-Saharan Africa. In 2010 53 million tons of maize were produced in sub-Saharan Africa on about one third of the total harvested cropland area (similar to 33 million ha). Our aim is to identify the limiting agroclimatic variable for maize growth and development in sub-Saharan Africa by analyzing the separated and combined effects of temperature and precipitation. Under changing climate, both climate variables are projected to change severely, and their impacts on crop yields are frequently assessed using process-based crop models. However it is often unclear which agroclimatic variable will have the strongest influence on crop growth and development under climate change and previous studies disagree over this question. We create synthetic climate data in order to study the effect of large changes in the length of the wet season and the amount of precipitation during the wet season both separately and in combination with changes in temperature. The dynamic global vegetation model for managed land LPJmL is used to simulate maize yields under current and future climatic conditions for the two 10-year periods 2056-2065 and 2081-2090 for three climate scenarios for the A1b emission scenario but without considering the beneficial CO2 fertilization effect. The importance of temperature and precipitation effects on maize yields varies spatially and we identify four groups of crop yield changes: regions with strong negative effects resulting from climate change (<-33% yield change), regions with moderate (-33% to -10% yield change) or slight negative effects (-10% to +6% yield change), and regions with positive effects arising from climate change mainly in currently temperature-limited high altitudes (>+6% yield change). In the first three groups temperature increases lead to maize yield reductions of 3 to 20%, with the exception of mountainous and thus cooler regions in South and East Africa. A reduction of the wet season precipitation causes decreases in maize yield of at least 30% and prevails over the effect of increased temperatures in southern parts of Mozambique and Zambia, the Sahel and parts of eastern Africa in the two projection periods. This knowledge about the limiting abiotic stress factor in each region will help to prioritize future research needs in modeling of agricultural systems as well as in drought and heat stress breeding programs and to identify adaption options in agricultural development projects. On the other hand the study enhances the understanding of temperature and water stress effects on crop yields in a global vegetation model in order to identify future research and model development needs. (C) 2013 Elsevier B.V. All rights reserved.