Abstract: Agronomic research is important to identify suitable options for improving soil carbon (C) sequestration and reducing soil CO2 emissions. Therefore, the objectives of this study were i) to analyse the on-farm effects of different nitrogen fertilization sources on soil respiration, ii) to explore the effect of fertilization on soil respiration sensitivity to soil temperature (T) and iii) to assess the effect of the different fertilization regimes on the soil C balance. We hypothesized that i) the soil CO2 emission dynamics in Mediterranean irrigated cropping systems were mainly affected by fertilization management and T and ii) fertilization affected the soil C budget via different C inputs and CO2 efflux. Four fertilization systems (farmyard manure, cattle slurry, cattle slurry + mineral, and mineral) were compared in a double-crop rotation based on silage maize (Zea mays L.) and a mixture of Italian ryegrass (Lolium multiflorum Lam.) and oats (Avena sativa L.). The research was performed in the dairy district of Arborea, in the coastal zone of Sardinia (Italy), from May 2011 to May 2012. The soil was a Psammentic Palexeralfs with a sandy texture (940 g sand kg− 1). The soil total respiration (SR), heterotrophic respiration (Rh), T and soil water content (SWC) were simultaneously measured in situ. The soil C balance was computed considering the Rh C losses and the soil C inputs from fertilizer and crop residues. The results showed that the maximum soil CO2 emission rates soon after the application of organic fertilizer reached values up to 12 μmol m− 2 s− 1. On average, the manure fertilizer showed significantly higher CO2 emissions, which resulted in a negative annual C balance (− 2.9 t ha− 1). T also affected the soil respiration temporal dynamics during the summer, consistently with results obtained in other temperate climatic regions that are characterized by wet summers and contrary to results from rainfed Mediterranean systems where the summer SR and Rh are constrained by the low SWC. The sensitivity of soil respiration to temperature significantly increased with C input from fertilizer. In conclusion, this research supported the hypotheses tested. Furthermore, the results indicated that i) soil CO2 efflux was significantly affected by fertilization management and T, and ii) fertilization with manure increased the soil respiration and resulted in a significantly negative soil C budget. This latter finding could be primarily explained by a reduction in productivity and, consequently, in crop residue with organic fertilization alone as compared to mineral, by the favourable SWC and T for mineralization, and by the sandy soil texture, which hindered the formation of macroaggregates and hence soil C stabilization, making fertilizer organic inputs highly susceptible to mineralization.

Abstract: The need to answer new scientific questions can be satisfied by an increased knowledge of physiological mechanisms which, in turn, can be used for improving the accuracy of simulations of process-based models. In this context, this report highlights areas that need to be further improved to facilitate the operational use of simulation models. It describes missing approaches within simulation models which, if implemented, would likely improve the representation of the dynamics of processes underlying different compartments of crop and grassland systems (e.g. plant growth and development, yield production, GHG emissions), as well as of the livestock production systems.  The following rationale has been used in the organization of this report. We first briefly introduced the need to improve the reliability of existing models. Then, we indicated climate change and its influence on the global carbon balance as the main issue to be addressed by existing crop and grassland (section 2), and livestock (section 3) models. In section 2, among the major aspects that if implemented may reduce the uncertainty inherent to model outputs, we suggested: i) quantifying the effects of climate extremes on biological systems; ii) modelling of multi-species sward; iii) coupling of pest and disease sub-models; iv) improvement of the carry-over effect. In section 3, as the most important aspects to consider in livestock models we indicated: i) impacts and dynamics of pathogens and disease; ii) heat stress effects on livestock; iii) effects on grassland productivity and nutritional values; iv) improvement of GHG emissions dynamics.  In Section 4, remarks are made concerning the need to implement the suggested aspects into the existing models.