Abstract: The accumulation of anthocyanins in plants exposed to salt stress has been largely documented. However, the functional link and regulatory components underlying the biosynthesis of these molecules during exposure to stress are largely unknown. In a screen of second site suppressors of the salt overly sensitive3-1 (sos3-1) mutant, we isolated the anthocyanin-impaired-response-1 (air1) mutant. air1 is unable to accumulate anthocyanins under salt stress, a key phenotype of sos3-1 under high NaCl levels (120 mM). The air1 mutant showed a defect in anthocyanin production in response to salt stress but not to other stresses such as high light, low phosphorous, high temperature or drought stress. This specificity indicated that air1 mutation did not affect anthocyanin biosynthesis but rather its regulation in response to salt stress. Analysis of this mutant revealed a T-DNA insertion at the first exon of an Arabidopsis thaliana gene encoding for a basic region-leucine zipper transcription factor. air1 mutants displayed higher survival rates compared to wild-type in oxidative stress conditions, and presented an altered expression of anthocyanin biosynthetic genes such as F3H, F3’H and LDOX in salt stress conditions. The results presented here indicate that AIR1 is involved in the regulation of various steps of the flavonoid and anthocyanin accumulation pathways and is itself regulated by the salt-stress response signalling machinery. The discovery and characterization of AIR1 opens avenues to dissect the connections between abiotic stress and accumulation of antioxidants in the form of flavonoids and anthocyanins.

Abstract: Local-scale daily climate scenarios are required for assessment of climate change impacts. ELPIS is a repository of local-scale climate scenarios for Europe, which are based on the LARS-WG weather generator and future projections from 2 multi-model ensembles, CMIP3 and EU-ENSEMBLES. In ELPIS, the site parameters for the 1980-2010 baseline scenarios were estimated by LARS-WG using daily weather from the European Crop Growth Monitoring System (CGMS) used in many European agricultural assessment studies. The objective of this paper was to compare ELPIS baseline scenarios with observed daily weather obtained independently from the European Climate Assessment (ECA) data set. Several statistical tests were used to compare distributions of climatic variables derived from ECA-observed daily weather and ELPIS-generated baseline scenarios. About 30% of selected sites have a difference in altitude of > 50 m compared with the CGMS grid-cell altitude that was selected to represent agricultural land within a grid-cell. Differences in altitude can explain significant Kolmogorov-Smirnov test (KS-test) results for distribution of daily temperature and in t-tests for temperature monthly means, because of the well-known negative correlation between temperature and elevation. For daily precipitation, the KS-test showed little difference between generated and observed data; however, the more sensitive t-test showed significant results for the sites where altitude differences were large. Approximately 11% of sites showed small positive or negative bias in monthly solar radiation, although 86% sites showed > 3 significant t-test results for monthly means. These results can be explained by differences in conversion of sunshine hours to solar radiation used in CGMS and LARS-WG. We conclude that, considering the limitations above, ELPIS baseline scenarios are suitable for agricultural impact assessments in Europe.

Abstract: Crop growth simulation models are increasingly used for regionally assessing the effects of climate change and variability on crop yields. These models require spatially and temporally detailed, location-specific, environmental (weather and soil) and management data as inputs, which are often difficult to obtain consistently for larger regions. Aggregating the resolution of input data for crop model applications may increase the uncertainty of simulations to an extent that is not well understood. The present study aims to systematically analyse the effect of changes in the spatial resolution of weather input data on yields simulated by four crop models (LINTUL-SLIM, DSSAT-CSM, EPIC and WOFOST) which were utilized to test possible interactions between weather input data resolution and specific modelling approaches representing different degrees of complexity. The models were applied to simulate grain yield of spring barley in Finland for 12 years between 1994 and 2005 considering five spatial resolutions of daily weather data: weather station (point) and grid-based interpolated data at resolutions of 10 km x 10 km; 20 km x 20 km; 50 km x 50 km and 100 km x 100 km. Our results show that the differences between models were larger than the effect of the chosen spatial resolution of weather data for the considered years and region. When displaying model results graphically, each model exhibits a characteristic ‘fingerprint’ of simulated yield frequency distributions. These characteristic distributions in response to the inter-annual weather variability were independent of the spatial resolution of weather input data. Using one model (LINTUL-SLIM), we analysed how the aggregation strategy, i.e. aggregating model input versus model output data, influences the simulated yield frequency distribution. Results show that aggregating weather data has a smaller effect on the yield distribution than aggregating simulated yields which causes a deformation of the model fingerprint. We conclude that changes in the spatial resolution of weather input data introduce less uncertainty to the simulations than the use of different crop models but that more evaluation is required for other regions with a higher spatial heterogeneity in weather conditions, and for other input data related to soil and crop management to substantiate our findings. Our results provide further evidence to support other studies stressing the importance of using not just one, but different crop models in climate assessment studies. (C) 2013 Elsevier B.V. All rights reserved.