Abstract: Climate change impact assessments are plagued with uncertainties from many sources, such as climate projections or the inadequacies in structure and parameters of the impact model. Previous studies tried to account for the uncertainty from one or two of these. Here, we developed a triple-ensemble probabilistic assessment using seven crop models, multiple sets of model parameters and eight contrasting climate projections together to comprehensively account for uncertainties from these three important sources. We demonstrated the approach in assessing climate change impact on barley growth and yield at Jokioinen, Finland in the Boreal climatic zone and Lleida, Spain in the Mediterranean climatic zone, for the 2050s. We further quantified and compared the contribution of crop model structure, crop model parameters and climate projections to the total variance of ensemble output using Analysis of Variance (ANOVA). Based on the triple-ensemble probabilistic assessment, the median of simulated yield change was -4% and +16%, and the probability of decreasing yield was 63% and 31% in the 2050s, at Jokioinen and Lleida, respectively, relative to 1981-2010. The contribution of crop model structure to the total variance of ensemble output was larger than that from downscaled climate projections and model parameters. The relative contribution of crop model parameters and downscaled climate projections to the total variance of ensemble output varied greatly among the seven crop models and between the two sites. The contribution of downscaled climate projections was on average larger than that of crop model parameters. This information on the uncertainty from different sources can be quite useful for model users to decide where to put the most effort when preparing or choosing models or parameters for impact analyses. We concluded that the triple-ensemble probabilistic approach that accounts for the uncertainties from multiple important sources provide more comprehensive information for quantifying uncertainties in climate change impact assessments as compared to the conventional approaches that are deterministic or only account for the uncertainties from one or two of the uncertainty sources.

Abstract: Ectopic cystatin expression has long been used in plant pest management, but the cysteine protease, targets of these inhibitors, might also have important functions in the control of plant lifespan and stress tolerance that remain poorly characterized. We therefore characterized the effects of expression of the rice cystatin, oryzacystatin-I (OCI), on the growth, development and stress tolerance of crop (soybean) and model (Arabidopsis thaliana) plants. Ectopic OCI expression in soybean enhanced shoot branching and leaf chlorophyll accumulation at later stages of vegetative development and enhanced seed protein contents and decreased the abundance of mRNAs encoding strigolactone synthesis enzymes. The OCI-expressing A. thaliana showed a slow-growth phenotype, with increased leaf numbers and enhanced shoot branching at flowering. The OCI-dependent inhibition of cysteine proteases enhanced drought tolerance in soybean and A. thaliana, photosynthetic CO2 assimilation being much less sensitive to drought-induced inhibition in the OCI-expressing soybean lines. Ectopic OCI expression or treatment with the cysteine protease inhibitor E64 increased lateral root densities in A. thaliana. E64 treatment also increased lateral root densities in the max2-1 mutants that are defective in strigolactone signalling, but not in the max3-9 mutants that are defective in strigolactone synthesis. Taken together, these data provide evidence that OCI-inhibited cysteine proteases participate in the control of growth and stress tolerance through effects on strigolactones. We conclude that cysteine proteases are important targets for manipulation of plant growth, development and stress tolerance, and also seed quality traits.

Abstract: The aim of the research was an agro-climatologic assessment of the amount of rainfall on a local scale, mainly aimed to identify trends in their changes and a possible rise in their variability over time. In the studies also we wanted to demonstrate the impact of the amount of rainfall in the region of Bydgoszcz on the yield of some crops. Material for the study consists of rainfall measurements, carried out in a stand- ard way in the years 1981-2010 at the Research Station of the University of Technology and Life Sciences in Bydgoszcz. Station is located in the village of Mochle, located approximately 20 km from the city centre (φ=53013’ N, λ=17051’E, h=98.5 m above sea level) in sparsely urbanized and industrialized area. We also used data of the yield of selected crops (potato, barley, corn for grain, legumes), from the production in the region of Kujawy and Pomorze as well as from our own experimental field. It has been shown that the average long-term rainfall during the growing season allows for classifying Bydgoszcz region as the area with the lowest rainfall in Poland. Analyzed rainfalls were characterized by a very high variability in time, resulting in climatic risk of plant growing. The largest temporal variability related to August. However, there was no extension of the time variability of rainfall totals in the period 1996-2010, as compared to the period 1981-1995. The sole significant growth trend during the period 1981-2010 was found in May. It appeared a tendency to a decline in summer rainfall totals (VI-VIII) in the annual rainfall total, which is consistent with the IPCC projections. Rainfall totals had highly signi cant impact on yields of selected crops. The highest correlation coefficients were found in relations crop-rainfall in the months of increased water needs of plants. Better correlations rainfall-crop were found using data from the production scale as compared with the scale of experimental field.