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Van Oosten, M. J., Sharkhuu, A., Batelli, G., Bressan, R. A., & Maggio, A. (2013). The Arabidopsis thaliana mutant air1 implicates SOS3 in the regulation of anthocyanins under salt stress. Plant Mol. Biol., 83(4-5), 405–415.
Abstract: The accumulation of anthocyanins in plants exposed to salt stress has been largely documented. However, the functional link and regulatory components underlying the biosynthesis of these molecules during exposure to stress are largely unknown. In a screen of second site suppressors of the salt overly sensitive3-1 (sos3-1) mutant, we isolated the anthocyanin-impaired-response-1 (air1) mutant. air1 is unable to accumulate anthocyanins under salt stress, a key phenotype of sos3-1 under high NaCl levels (120 mM). The air1 mutant showed a defect in anthocyanin production in response to salt stress but not to other stresses such as high light, low phosphorous, high temperature or drought stress. This specificity indicated that air1 mutation did not affect anthocyanin biosynthesis but rather its regulation in response to salt stress. Analysis of this mutant revealed a T-DNA insertion at the first exon of an Arabidopsis thaliana gene encoding for a basic region-leucine zipper transcription factor. air1 mutants displayed higher survival rates compared to wild-type in oxidative stress conditions, and presented an altered expression of anthocyanin biosynthetic genes such as F3H, F3’H and LDOX in salt stress conditions. The results presented here indicate that AIR1 is involved in the regulation of various steps of the flavonoid and anthocyanin accumulation pathways and is itself regulated by the salt-stress response signalling machinery. The discovery and characterization of AIR1 opens avenues to dissect the connections between abiotic stress and accumulation of antioxidants in the form of flavonoids and anthocyanins.
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Semenov, M. A., Pilkington-Bennett, S., & Calanca, P. (2013). Validation of ELPIS 1980-2010 baseline scenarios using the observed European Climate Assessment data set. Clim. Res., 57(1), 1–9.
Abstract: Local-scale daily climate scenarios are required for assessment of climate change impacts. ELPIS is a repository of local-scale climate scenarios for Europe, which are based on the LARS-WG weather generator and future projections from 2 multi-model ensembles, CMIP3 and EU-ENSEMBLES. In ELPIS, the site parameters for the 1980-2010 baseline scenarios were estimated by LARS-WG using daily weather from the European Crop Growth Monitoring System (CGMS) used in many European agricultural assessment studies. The objective of this paper was to compare ELPIS baseline scenarios with observed daily weather obtained independently from the European Climate Assessment (ECA) data set. Several statistical tests were used to compare distributions of climatic variables derived from ECA-observed daily weather and ELPIS-generated baseline scenarios. About 30% of selected sites have a difference in altitude of > 50 m compared with the CGMS grid-cell altitude that was selected to represent agricultural land within a grid-cell. Differences in altitude can explain significant Kolmogorov-Smirnov test (KS-test) results for distribution of daily temperature and in t-tests for temperature monthly means, because of the well-known negative correlation between temperature and elevation. For daily precipitation, the KS-test showed little difference between generated and observed data; however, the more sensitive t-test showed significant results for the sites where altitude differences were large. Approximately 11% of sites showed small positive or negative bias in monthly solar radiation, although 86% sites showed > 3 significant t-test results for monthly means. These results can be explained by differences in conversion of sunshine hours to solar radiation used in CGMS and LARS-WG. We conclude that, considering the limitations above, ELPIS baseline scenarios are suitable for agricultural impact assessments in Europe.
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Sanna, M., Bellocchi, G., Fumagalli, M., & Acutis, M. (2015). A new method for analysing the interrelationship between performance indicators with an application to agrometeorological models. Env. Model. Softw., 73, 286–304.
Abstract: The use of a variety of metrics is advocated to assess model performance but correlated metrics may convey the same information, thus leading to redundancy. Starting from this assumption, a method was developed for selecting, from among a collection of performance indicators, one or more subsets providing the same information as the entire set. The method, based on the definition of “stable correlation”, was applied to 23 performance indicators of agrometeorological models, calculated on large sets of simulated and observed data of four agronomic and meteorological variables: above-ground biomass, leaf area index, hourly air relative humidity and daily solar radiation. Two subsets were determined: {Squared Bias, Root Mean Squared Relative Error, Coefficient of Determination, Pattern Index, Modified Modelling Efficiency}, {Persistence Model Efficiency, Root Mean Squared Relative Error, Coefficient of Determination, Pattern Index}. The method needs corroboration but is statistically founded and can support the implementation of standardized evaluation tools. (C) 2015 Elsevier Ltd. All rights reserved.
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Legarrea, S., Betancourt, M., Plaza, M., Fraile, A., García-Arenal, F., & Fereres, A. (2012). Dynamics of nonpersistent aphid-borne viruses in lettuce crops covered with UV-absorbing nets. Virus Res., 165(1), 1–8.
Abstract: Aphid-transmitted viruses frequently cause severe epidemics in lettuce grown under Mediterranean climates. Spatio-temporal dynamics of aphid-transmitted viruses and its vector were studied on lettuce (Lactuca sativa L.) grown under tunnels covered by two types of nets: a commercial UV-absorbing net (Bionet) and a Standard net. A group of plants infected by Cucumber mosaic virus (CMV, family Bromoviridae, genus Cucumovirus) and Lettuce mosaic virus (LMV, family Potyviridae, genus Potyvirus) was transplanted in each plot. The same virus-infected source plants were artificially infested by the aphid Macrosiphum euphorbiae (Thomas). Secondary spread of insects was weekly monitored and plants were sampled for the detection of viruses every two weeks. In 2008, the infection rate of both CMV and LMV were lower under the Bionet than under the Standard cover, probably due to the lower population density and lower dispersal rate achieved by M. euphorbiae. However, during spring of 2009, significant differences in the rate of infection between the two covers were only found for LMV six weeks after transplant. The spatial distribution of the viruses analysed by SADIE methodology was “at random”, and it was not associated to the spatial pattern of the vector. The results obtained are discussed analyzing the wide range of interactions that occurred among UV-radiation, host plant, viruses, insect vector and environmental conditions. Our results show that UV-absorbing nets can be recommended as a component of an integrated disease management program to reduce secondary spread of lettuce viruses, although not as a control measure on its own.
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Eza, U., Shtiliyanova, A., Borras, D., Bellocchi, G., Carrère, P., & Martin, R. (2015). An open platform to assess vulnerabilities to climate change: An application to agricultural systems. Ecological Informatics, 30, 389–396.
Abstract: Numerous climate futures are now available from global climate models. Translation of climate data such as precipitation and temperatures into ecologically meaningful outputs for managers and planners is the next frontier. We describe a model-based open platform to assess vulnerabilities of agricultural systems to climate change on pixel-wise data. The platform includes a simulation modeling engine and is suited to work with NetCDF format of input and output files. In a case study covering a region (Auvergne) in the Massif Central of France, the platform is configured to characterize climate (occurrence of arid conditions in historical and projected climate records), soils and human management, and is then used to assess the vulnerability to climate change of grassland productivity (downscaled to a fine scale). We demonstrate how using climate time series, and process-based simulations vulnerabilities can be defined at fine spatial scales relevant to farmers and land managers, and can be incorporated into management frameworks. (C) 2015 Elsevier B.V. All rights reserved.
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