Abstract: Agricultural nitrous oxide (N2O) pollution resulting from the use of synthetic fertilizers represents a significant contribution to anthropogenic greenhouse gas emissions, providing a rationale for reduced use of nitrogen (N) fertilizers. Nitrogen limitation results in extensive systems rebalancing that remodels metabolism and defence processes. To analyse the regulation underpinning these responses, barley (Horedeum vulgare) seedlings were grown for 7 d under N-deficient conditions until net photosynthesis was 50% lower than in N-replete controls. Although shoot growth was decreased there was no evidence for the induction of oxidative stress despite lower total concentrations of N-containing antioxidants. Nitrogen-deficient barley leaves were rich in amino acids, sugars and tricarboxylic acid cycle intermediates. In contrast to N-replete leaves one-day-old nymphs of the green peach aphid (Myzus persicae) failed to reach adulthood when transferred to N-deficient barley leaves. Transcripts encoding cell, sugar and nutrient signalling, protein degradation and secondary metabolism were over-represented in N-deficient leaves while those associated with hormone metabolism were similar under both nutrient regimes with the exception of mRNAs encoding proteins involved in auxin metabolism and responses. Significant similarities were observed between the N-limited barley leaf transcriptome and that of aphid-infested Arabidopsis leaves. These findings not only highlight significant similarities between biotic and abiotic stress signalling cascades but also identify potential targets for increasing aphid resistance with implications for the development of sustainable agriculture.

Abstract: A field experiment was conducted on Haplic Luvisol developed from loess to assess the effects of soil deformation and straw mulch on soil water status (matric potential), temperature, penetration resistance, soybean growth, seed yield and yield components including straw, protein and oil in 2006-2008. Water use efficiencies related to the amount of rainfall during the growing seasons were calculated for seeds and total above ground biomass. The soil deformation levels (main plots) comprised the following trials: non-compacted (NC, 0 tractor pass), moderately compacted (MC, 3 passes), and strongly compacted (SC, 5 passes). A uniform seedbed in all plots was prepared by harrowing before planting. The main plots included sub-plots without and with surface wheat straw mulch (0.5 kg m(-2)) and the corresponding trials were NC + M, MC + M, SC + M. The amount and distribution of rainfall during the growing season differed among the experimental years with extended drought at bloom-full seed (R2-R6) stages in 2006, good water supply in 2007, and alternative periods with relatively high and low rainfalls in 2008. The effect of soil deformation on matric potential was influenced by weather conditions, soybean growth phase, mulching and depth. The differences were greatest in 2007 and 2008 at R7-R8 growth stages. With increasing deformation level from NC to SC matric potential for 0-15 cm depth during these stages significantly decreased from -401 to -1184 kPa in 2007 and from -1154 to -1432 kPa in 2008. On mulched soil, the corresponding ranges were from -541 to -841 klpa and from -748 to -1386 kPa, respectively. In the dry summer 2006, the differences were smaller and less consistent. Irrespective of soil deformation level, mulching reduced soil temperature in most growth phases but most pronounced initially. Most yield components increased from NC to MC during the experiments which could be attributed to enhanced root water and nutrient uptake rates and decreased from MC to SC due to high soil strength that restrained root growth down to deeper depth. The yields of seeds, straw, protein and oil as well as water productivity of soybean seed and biomass were improved by mulching in 2007-2008. This improvement was more pronounced in 2007 when the mean yield of seeds, protein and oil were significantly greater by 16, 29 and 11%, respectively and was attributed to positive alterations in soil water retention. These results indicate the possibilities of improvement in soybean performance by identifying allowable amount of traffic and mulching practices at planting depending on weather fluctuations during the growing season. Since rainfall and air temperature distribution in 2007 are close to those averaged over a long period of time, the use of straw mulch may positively affect soybean performance and yields excluding anomalously dry years. The positive effect of straw mulch can be enhanced by moderate soil deformation combined with seedbed loosening before planting to avoid constraining effect of soil structure on crop establishment. (C) 2015 Elsevier B.V. All rights reserved.