Abstract: Increasing crop productivity while simultaneously reducing the environmental footprint of crop production is considered a major challenge for the coming decades. Even short episodes of heat stress can reduce crop yield considerably causing low resource use efficiency. Studies on the impact of heat stress on crop yields over larger regions generally rely on temperatures measured by standard weather stations at 2 m height. Canopy temperatures measured in this study in field plots of rye were up to 7 degrees C higher than air temperature measured at typical weather station height with the differences in temperatures controlled by soil moisture contents. Relationships between heat stress and grain number derived from controlled environment studies were only confirmed under field conditions when canopy temperature was used to calculate stress thermal time. By using hourly mean temperatures measured by 78 weather stations located across Germany for the period 1994-2009 it is estimated, that mean yield declines in wheat due to heat stress during flowering were 0.7% when temperatures are measured at 2 m height, but yield declines increase to 22% for temperatures measured at the ground. These results suggest that canopy temperature should be simulated or estimated to reduce uncertainty in assessing heat stress impacts on crop yield.

Abstract: The human population is projected to reach more than 10 billion in the year 2100. Together with changing consumption pattern, population growth will lead to increasing food demand. The question arises whether or not the Earth is capable of fulfilling this demand. In this study, we approach this question by estimating the carrying capacity of current agricultural systems (K-C), which does not measure the maximum number of people the Earth is likely to feed in the future, but rather allows for an indirect assessment of the increases in agricultural productivity required to meet demands. We project agricultural food production under progressing climate change using the state-of-the-art dynamic global vegetation model LPJmL, and input data of 3 climate models. For 1990 to 2100 the worldwide annual caloric yield of the most important 11 crop types is simulated. Model runs with and without elevated atmospheric CO2 concentrations are performed in order to investigate CO2 fertilization effects. Country-specific per-capita caloric demands fixed at current levels and changing demands based on future GDP projections are considered to assess the role of future dietary shifts. Our results indicate that current population projections may considerably exceed the maximum number of people that can be fed globally if climate change is not accompanied by significant changes in land use, agricultural efficiencies and/or consumption pathways. We estimate the gap between projected population size and K-C to reach 2 to 6.8 billion people by 2100. We also present possible caloric self-supply changes between 2000 and 2100 for all countries included in this study. The results show that predominantly developing countries in tropical and subtropical regions will experience vast decreases of self-supply. Therefore, this study is important for planning future large-scale agricultural management, as well as the critical assessment of population projections, which should take food-mediated climate change feedbacks into account

Abstract: Ectopic cystatin expression has long been used in plant pest management, but the cysteine protease, targets of these inhibitors, might also have important functions in the control of plant lifespan and stress tolerance that remain poorly characterized. We therefore characterized the effects of expression of the rice cystatin, oryzacystatin-I (OCI), on the growth, development and stress tolerance of crop (soybean) and model (Arabidopsis thaliana) plants. Ectopic OCI expression in soybean enhanced shoot branching and leaf chlorophyll accumulation at later stages of vegetative development and enhanced seed protein contents and decreased the abundance of mRNAs encoding strigolactone synthesis enzymes. The OCI-expressing A. thaliana showed a slow-growth phenotype, with increased leaf numbers and enhanced shoot branching at flowering. The OCI-dependent inhibition of cysteine proteases enhanced drought tolerance in soybean and A. thaliana, photosynthetic CO2 assimilation being much less sensitive to drought-induced inhibition in the OCI-expressing soybean lines. Ectopic OCI expression or treatment with the cysteine protease inhibitor E64 increased lateral root densities in A. thaliana. E64 treatment also increased lateral root densities in the max2-1 mutants that are defective in strigolactone signalling, but not in the max3-9 mutants that are defective in strigolactone synthesis. Taken together, these data provide evidence that OCI-inhibited cysteine proteases participate in the control of growth and stress tolerance through effects on strigolactones. We conclude that cysteine proteases are important targets for manipulation of plant growth, development and stress tolerance, and also seed quality traits.