Abstract: Ultraviolet (UV) radiation directly regulates a multitude of herbivore life processes, in addition to indirectly affecting insect success via changes in plant chemistry and morphogenesis. Here we looked at plant and insect (aphid and whitefly) exposure to supplemental UV-A radiation in the glasshouse environment and investigated effects on insect population growth. Glasshouse grown peppers and eggplants were grown from seed inside cages covered by novel plastic filters, one transparent and the other opaque to UV-A radiation. At a 10-true leaf stage for peppers (53 days) and 4-true leaf stage for eggplants (34 days), plants were harvested for chemical analysis and infested by aphids and whiteflies, respectively. Clip-cages were used to introduce and monitor the insect fitness and populations of the pests studied. Insect pre-reproductive period, fecundity, fertility and intrinsic rate of natural increase were assessed. Crop growth was monitored weekly for 7 and 12 weeks throughout the crop cycle of peppers and eggplants, respectively. At the end of the insect fitness experiment, plants were harvested (68 days and 18-true leaf stage for peppers, and 104 days and 12-true leaf stage for eggplants) and leaves analysed for secondary metabolites, soluble carbohydrates, amino acids, total proteins and photosynthetic pigments. Our results demonstrate for the first time, that UV-A modulates plant chemistry with implications for insect pests. Both plant species responded directly to UV-A by producing shorter stems but this effect was only significant in pepper whilst UV-A did not affect the leaf area of either species. Importantly, in pepper, the UV-A treated plants contained higher contents of secondary metabolites, leaf soluble carbohydrates, free amino acids and total content of protein. Such changes in tissue chemistry may have indirectly promoted aphid performance. For eggplants, chlorophylls a and b, and carotenoid levels decreased with supplemental UV-A over the entire crop cycle but UV-A exposure did not affect leaf secondary metabolites. However, exposure to supplemental UV-A had a detrimental effect on whitefly development, fecundity and fertility presumably not mediated by plant cues as compounds implied in pest nutrition – proteins and sugars – were unaltered.

Abstract: To reduce the dependence on local expert knowledge, which is important for large-scale crop modelling studies, we analyzed sowing dates and rules for maize (Zea mays L.) and sorghum (Sorghum bicolor (L)) at three locations in Burkina Faso with strongly decreasing rainfall amounts from south to north. We tested in total 22 methods to derive optimal sowing dates that result in highest water-limited yields and lowest yield variation in a reproducible and objective way. The WOFOST crop growth simulation model was used. We found that sowing dates that are based on local expert knowledge, may work quite well for Burkina Faso and for West Africa in general. However, when no a priori information is available, maize should be sown between Julian days 160 and 200, with application of the following criteria: (a) cumulative rainfall in the sowing window is >= 3 cm or available soil moisture content is >2 cm in the moderately dry central part of Burkina Faso, (b) cumulative rainfall in this period is >= 2 cm or available soil moisture content is >1 cm in the more humid regions in the southern part of Burkina Faso. Sorghum should also be sown between Julian days 160 and 200 with application of the following criteria: (a) in the dry northern part of Burkina Faso the long duration sorghum variety should be sown when cumulative rainfall is >2 cm in the sowing window, and the short duration sorghum variety should be sown later when cumulative rainfall is >= 3 cm, (b) in central Burkina Faso sowing should start when cumulative rainfall in this period is >= 2 cm or when available soil moisture content is >1 cm. Sowing date rules are shown to be generally crop and location specific and are not generic for West Africa. However, the required precision of the sowing rules appears to rapidly decrease with increasing duration and intensity of the rainy season. Sowing delay as a result of, for example, labour constraints, has a disastrous effect on rainfed maize and sorghum yields, particularly in the northern part of West Africa with low rainfall. Optimization of sowing dates can also be done by simulating crop yields in a time window of two months around a predefined sowing date. Using these optimized dates appears to result in a good estimate of the maximal mean rainfed yield level. (C) 2015 Elsevier B.V. All rights reserved.

Abstract: Seeds are important to agriculture and conservation of plant biodiversity. In agriculture, seed germination performance is an important determinant of crop yield, in particular under adverse climatic conditions. Deterioration in seed quality is associated with the accumulation of cellular damage to macromolecules including lipids, protein, and DNA. Mechanisms that mitigate the deleterious cellular damage incurred in the quiescent state and in cycles of desiccation-hydration are crucial for the maintenance of seed viability and germination vigour. In early-imbibing seeds, damage to the embryo genome must be repaired prior to initiation of cell division to minimize growth inhibition and mutation of genetic information. Here we review recent advances that have established molecular links between genome integrity and seed quality. These studies identified that maintenance of genome integrity is particularly important to the seed stage of the plant lifecycle, revealing new insight into the physiological roles of plant DNA repair and recombination mechanisms. The high conservation of DNA repair and recombination factors across plant species underlines their potential as promising targets for the improvement of crop performance and development of molecular markers for prediction of seed vigour.