Abstract: Phytocystatins are a well-characterized class of naturally occurring protease inhibitors that function by preventing the catalysis of papain-like cysteine proteases. The action of cystatins in biotic stress resistance has been studied intensively, but relatively little is known about their functions in plant growth and defence responses to abiotic stresses, such as drought. Extreme weather events, such as drought and flooding, will have negative impacts on the yields of crop plants, particularly grain legumes. The concepts that changes in cellular protein content and composition are required for acclimation to different abiotic stresses, and that these adjustments are achieved through regulation of proteolysis, are widely accepted. However, the nature and regulation of the protein turnover machinery that underpins essential stress-induced cellular restructuring remain poorly characterized. Cysteine proteases are intrinsic to the genetic programmes that underpin plant development and senescence, but their functions in stress-induced senescence are not well defined. Transgenic plants including soybean that have been engineered to constitutively express phytocystatins show enhanced tolerance to a range of different abiotic stresses including drought, suggesting that manipulation of cysteine protease activities by altered phytocystatin expression in crop plants might be used to improve resilience and quality in the face of climate change.

Abstract: Radiant frost is a significant production constraint to wheat (Triticum aestivum) and barley (Hordeum vulgare), particularly in regions where spring-habit cereals are grown through winter, maturing in spring. However, damage to winter-habit cereals in reproductive stages is also reported. Crops are particularly susceptible to frost once awns or spikes emerge from the protection of the flag leaf sheath. Post-head-emergence frost (PHEF) is a problem distinct from other cold-mediated production constraints. To date, useful increased PHEF resistance in cereals has not been identified. Given the renewed interest in reproductive frost damage in cereals, it is timely to review the problem. Here we update the extent and impacts of PHEF and document current management options to combat this challenge. We clarify terminology useful for discussing PHEF in relation to chilling and other freezing stresses. We discuss problems characterizing radiant frost, the environmental conditions leading to PHEF damage, and the effects of frost at different growth stages. PHEF resistant cultivars would be highly desirable, to both reduce the incidence of direct frost damage and to allow the timing of crop maturity to be managed to maximize yield potential. A framework of potential adaptation mechanisms is outlined. Clarification of these critical issues will sharpen research focus, improving opportunities to identify genetic sources for improved PHEF resistance.

Abstract: The aim of this study is to compare the water and organic material balance, yields and other aspects estimated within crop rotations by the Hermes crop model for present and future climatic conditions in the Czech Republic. Moreover, this is a pilot study for the complex and continuous crop rotations modeling (using both single crop models and ensembles) in connection with transient climate change scenarios. For this purpose, three locations representing important agricultural regions of the Czech Republic (with different climatic conditions) were selected. The crop rotation (including spring barley, silage maize, winter wheat, winter rape, and winter wheat in the listed order) was simulated from 1981-2080. The period 1981-2010 was covered by measured meteorological data, and the period 2011-2080 was represented by a transient synthetic weather series from the weather generator M&Rfi. The generated data was based on five circulation models representing an ensemble of 18 CMIP3 global circulation models to preserve to a large degree the uncertainty of the original ensemble. Two types of crop management were compared, and the influences of soil quality, increasing atmospheric CO2 and magnitude of adaptation measure (in the form of sowing date changes) were also considered. According to the results, if a “dry” scenario (such as GFCM21) would occur, then all the C3 crops produced in drier regions would be devastated in a significant number of seasons; for example, by the 2070s, up to 19.5%, 21.5% and 47.0% of seasons with winter rape, spring barley and winter wheat, respectively, would have a yield level below 50% of the present yield. Negative impacts are likely even on premium-quality soils regardless of the use of a flexible sowing date and accounting for increasing CO2 concentrations. Moreover, in some cases, the use of catch crops can have negative impacts, exacerbating the soil water deficit for the subsequent crops. This study (submitted to Climate Research journal) will be used as a pilot for subsequent activities. In this area, following calculations (the same set of stations and updated climate scenarios) using growth models ensemble (currently includes 12 modeling approaches) started to estimate uncertainty aspects. Consequently, the analysis within wider range of conditions (across continents) and farming methods will be conducted. No Label