Abstract: • ICFAR-DB organises and stores data from 16 Italian long term agronomic experiments. • ICFAR-DB fulfils interoperability using system dynamics ontology and AgMIP nomenclature. • ICFAR information management system moves closer data to model and vice versa. The IC-FAR national project (Linking long term observatories with crop system modelling for better understanding of climate change impact, and adaptation strategies for Italian cropping systems) initiated in 2013 with the primary aim of implementing data from 16 long term Italian agronomic experiments in a common, interoperable structure. The building of a common database (DB) structure demands a harmonization process aimed at standardising concepts, language and data in order to make them clear, and has to produce a well-documented and easily available tool for the whole scientific community. The Agricultural Model Intercomparison and Improvement Project (AgMIP) has made a great effort in this sense, improving the vocabulary developed by the International Consortium for Agricultural Systems Applications (ICASA) and defining harmonization procedures. Nowadays, these ones have also to be addressed to facilitate the extraction of input files for crop model simulations. Substantially, two alternative directions can be pursued: adapting data to models, building a standard storage structure and using translators that convert DB information to model input files; or adapting models to data, using the same storage structure for feeding modelling solutions constituted by combining model components, re-implemented in the same model platform. The ICFAR information management system simplifies data entry, improves model input extraction (implementing System Dynamics ontology), and satisfies both the paradigms. This has required the development of different software tools: ICFAR-DB for data entry and storage; a model input extractor for feeding the crop models (MoLInEx); SEMoLa platform for building modelling solutions and performing via scripts the model intercomparison. The use of the standard AgMIP/ICASA nomenclature in the ICFAR-DB and the opportunity to create files with MoLInex for feeding AgMIP model translators allow full system interoperability.

Abstract: Successive single day transfers of pot-grown wheat to high temperature (35/30°C day/night) replicated controlled environments, from the second node detectable to the milky-ripe growth stages, provides the strongest available evidence that the fertility of wheat can be highly vulnerable to heat stress during two discrete peak periods of susceptibility: early booting [decimal growth stage (GS) 41-45] and early anthesis (GS 61-65). A double Gaussian fitted simultaneously to grain number and weight data from two contrasting elite lines (Renesansa, listed in Serbia, Ppd-D1a, Rht8; Savannah, listed in UK, Ppd-D1b, Rht-D1b) identified peak periods of main stem susceptibility centered on 3 (s.e. = 0.82) and 18 (s.e. = 0.55) days (mean daily temperature = 14.3°C) pre-GS 65 for both cultivars. Severity of effect depended on genotype, growth stage and their interaction: grain set relative to that achieved at 20/15°C dropped below 80% for Savannah at booting and Renesansa at anthesis. Savannah was relatively tolerant to heat stress at anthesis. A further experiment including 62 lines of the mapping, doubled-haploid progeny of Renesansa × Savannah found tolerance at anthesis to be associated with Ppd-D1b, Rht-D1b, and a QTL from Renesansa on chromosome 2A. None of the relevant markers were associated with tolerance during booting. Rht8 was never associated with heat stress tolerance, a lack of effect confirmed in a further experiment where Rht8 was included in a comparison of near isogenic lines in a cv. Paragon background. Some compensatory increases in mean grain weight were observed, but only when stress was applied during booting and only where Ppd-D1a was absent.