Abstract: Ultraviolet (UV) radiation, particularly in the UV-A + B range (280-400 nm) is a fraction of the solar spectrum that regulates almost every aspect of insect behaviour, including orientation towards hosts, alighting, arrestment and feeding behaviour. To study the role of UV radiation on the flight activity of five insect species of agricultural importance (pests Myzus persicae, Bemisia tabaci and Tuta absoluta, and natural enemies Aphidius colemani and Sphaerophoria rueppellii), one-chamber tunnels were covered with six cladding materials with different light transmittance properties ranging from 2% to 83% UV and 54% to 85% photosynthetically active radiation (PAR). Inside each tunnel, insects were released from tubes placed in a platform suspended from the ceiling. Specific targets varying with insect species were placed at different distances from the platform. Evaluation parameters were designed for each insect and tested separately. The ability of insects to leave the platform was assessed, as well as the number of captures, eggs or mummies in each target, either sticky traps or plants. Our results suggest differences in flight activity among insect species and UV-blocking nets. The UV-opaque film drastically prevented aphids, and whiteflies from flying outside the tubes whereas T. absoluta, syrphids and parasitoids were not affected. Aphid flight behaviour was affected by the UV-opaque film compared to the other nets, especially in the furthest target of the tunnel. Fewer aphids reached distant traps under UV-absorbing nets, and significantly more aphids could fly to the end of tunnels covered with non-UV-blocking materials. Orientation of B. tabaci and T. absoluta was also negatively affected by the UV-opaque film although in a different trend. Unlike aphids, differences in B. tabaci captures were mainly found in the closest targets. UV transmittance did not have any effects on parasitoids, and S. rueppellii, implying cues other than visual for these insects under our experimental conditions. Further effects of photoselective enclosures on greenhouse pests and their natural enemies are discussed.

Abstract: Polyphenol oxidase (PPO) catalyses the oxidation of monophenols and/or o-diphenols to o-quinones with the concomitant reduction of oxygen to water which results in protein complexing and the formation of brown melanin pigments. The most frequently suggested role for PPO in plants has been in defence against herbivores and pathogens, based on the physical separation of the chloroplast-localized enzyme from the vacuole-localized substrates. The o-quinone-protein complexes, formed as a consequence of cell damage, may reduce the nutritional value of the tissue and thereby reduce predation but can also participate in the formation of structural barriers against invading pathogens. However, since a sufficient level of compartmentation-based regulation could be accomplished if PPO was targeted to the cytosol, the benefit derived by some plant species in having PPO present in the chloroplast lumen remains an intriguing question. So is there more to the chloroplastic location of PPO? An interaction between PPO activity and photosynthesis has been proposed on more than one occasion but, to date, evidence either for or against direct involvement has been equivocal, and the lack of identified chloroplastic substrates remains an issue. Similarly, PPO has been suggested to have both pro- and anti-oxidant functions. Nevertheless, several independent lines of evidence suggest that PPO responds to environmental conditions and could be involved in the response of plants to abiotic stress. This review highlights our current understanding of the in vivo functions of PPO and considers the potential opportunities it presents for exploitation to increase stress tolerance in food crops.

Abstract: The ‘phosphorus problem’ has recently received strong interest with two distinct strands of importance. The first is that too much phosphorus (P) is entering into waste water, creating a significant economic and ecological problem. Secondly, while agricultural demand for phosphate fertilizer is increasing to maintain crop yields, rock phosphate reserves are rapidly declining. Unravelling the mechanisms by which plants sense, respond to, and acquire phosphate can address both problems, allowing the development of crop plants that are more efficient at acquiring and using limited amounts of phosphate while at the same time improving the potential of plants and other photosynthetic organisms for nutrient recapture and recycling from waste water. In this review, we attempt to synthesize these important but often disparate parts of the debate in a holistic fashion, since solutions to such a complex problem require integrated and multidisciplinary approaches that address both P supply and demand. Rapid progress has been made recently in our understanding of local and systemic signalling mechanisms for phosphate, and of expression and regulation of membrane proteins that take phosphate up from the environment and transport it within the plant. We discuss the current state of understanding of such mechanisms involved in sensing and responding to phosphate stress. We also discuss approaches to improve the P-use efficiency of crop plants and future direction for sustainable use of P, including use of photosynthetic organisms for recapture of P from waste waters.