Abstract: Ectopic cystatin expression has long been used in plant pest management, but the cysteine protease, targets of these inhibitors, might also have important functions in the control of plant lifespan and stress tolerance that remain poorly characterized. We therefore characterized the effects of expression of the rice cystatin, oryzacystatin-I (OCI), on the growth, development and stress tolerance of crop (soybean) and model (Arabidopsis thaliana) plants. Ectopic OCI expression in soybean enhanced shoot branching and leaf chlorophyll accumulation at later stages of vegetative development and enhanced seed protein contents and decreased the abundance of mRNAs encoding strigolactone synthesis enzymes. The OCI-expressing A. thaliana showed a slow-growth phenotype, with increased leaf numbers and enhanced shoot branching at flowering. The OCI-dependent inhibition of cysteine proteases enhanced drought tolerance in soybean and A. thaliana, photosynthetic CO2 assimilation being much less sensitive to drought-induced inhibition in the OCI-expressing soybean lines. Ectopic OCI expression or treatment with the cysteine protease inhibitor E64 increased lateral root densities in A. thaliana. E64 treatment also increased lateral root densities in the max2-1 mutants that are defective in strigolactone signalling, but not in the max3-9 mutants that are defective in strigolactone synthesis. Taken together, these data provide evidence that OCI-inhibited cysteine proteases participate in the control of growth and stress tolerance through effects on strigolactones. We conclude that cysteine proteases are important targets for manipulation of plant growth, development and stress tolerance, and also seed quality traits.

Abstract: We compared the precision of simple random sampling (SimRS) and seven types of stratified random sampling (StrRS) schemes in estimating regional mean of water-limited yields for two crops (winter wheat and silage maize) that were simulated by fourteen crop models. We found that the precision gains of StrRS varied considerably across stratification methods and crop models. Precision gains for compact geographical stratification were positive, stable and consistent across crop models. Stratification with soil water holding capacity had very high precision gains for twelve models, but resulted in negative gains for two models. Increasing the sample size monotonously decreased the sampling errors for all the sampling schemes. We conclude that compact geographical stratification can modestly but consistently improve the precision in estimating regional mean yields. Using the most influential environmental variable for stratification can notably improve the sampling precision, especially when the sensitivity behavior of a crop model is known.

Abstract: Climate change is expected to affect optimum agricultural management practices for autumn-sown wheat, especially those related to sowing date and nitrogen (N) fertilization. To assess the direction and quantity of these changes for an important production region in eastern Austria, the agricultural production systems simulator was parameterized, evaluated and subsequently used to predict yield production and grain protein content under current and future conditions. Besides a baseline climate (BL, 1981–2010), climate change scenarios for the period 2035–65 were derived from three Global Circulation Models (GCMs), namely CGMR, IPCM4 and MPEH5, with two emission scenarios, A1B and B1. Crop management scenarios included a combination of three sowing dates (20 September, 20 October, 20 November) with four N fertilizer application rates (60, 120, 160, 200 kg/ha). Each management scenario was run for 100 years of stochastically generated daily weather data. The model satisfactorily simulated productivity as well as water and N use of autumn- and spring-sown wheat crops grown under different N supply levels in the 2010/11 and 2011/12 experimental seasons. Simulated wheat yields under climate change scenarios varied substantially among the three GCMs. While wheat yields for the CGMR model increased slightly above the BL scenario, under IPCM4 projections they were reduced by 29 and 32% with low or high emissions, respectively. Wheat protein appears to increase with highest increments in the climate scenarios causing the largest reductions in grain yield (IPCM4 and MPEH-A1B). Under future climatic conditions, maximum wheat yields were predicted for early sowing (September 20) with 160 kg N/ha applied at earlier dates than the current practice.