Abstract: This paper deals with the problem of predicting biomass and grain protein content using improved particle filtering (IPF) based on minimizing the Kullback-Leibler divergence. The performances of IPF are compared with those of the conventional particle filtering (PF) in two comparative studies. In the first one, we apply IPF and PF at a simple dynamic crop model with the aim to predict a single state variable, namely the winter wheat biomass, and to estimate several model parameters. In the second study, the proposed IPF and the PF are applied to a complex crop model (AZODYN) to predict a winter-wheat quality criterion, namely the grain protein content. The results of both comparative studies reveal that the IPF method provides a better estimation accuracy than the PF method. The benefit of the IPF method lies in its ability to provide accuracy related advantages over the PF method since, unlike the PF which depends on the choice of the sampling distribution used to estimate the posterior distribution, the IPF yields an optimum choice of this sampling distribution, which also utilizes the observed data. The performance of the proposed method is evaluated in terms of estimation accuracy, root mean square error, mean absolute error and execution times.

Abstract: Polyphenol oxidase (PPO) catalyses the oxidation of monophenols and/or o-diphenols to o-quinones with the concomitant reduction of oxygen to water which results in protein complexing and the formation of brown melanin pigments. The most frequently suggested role for PPO in plants has been in defence against herbivores and pathogens, based on the physical separation of the chloroplast-localized enzyme from the vacuole-localized substrates. The o-quinone-protein complexes, formed as a consequence of cell damage, may reduce the nutritional value of the tissue and thereby reduce predation but can also participate in the formation of structural barriers against invading pathogens. However, since a sufficient level of compartmentation-based regulation could be accomplished if PPO was targeted to the cytosol, the benefit derived by some plant species in having PPO present in the chloroplast lumen remains an intriguing question. So is there more to the chloroplastic location of PPO? An interaction between PPO activity and photosynthesis has been proposed on more than one occasion but, to date, evidence either for or against direct involvement has been equivocal, and the lack of identified chloroplastic substrates remains an issue. Similarly, PPO has been suggested to have both pro- and anti-oxidant functions. Nevertheless, several independent lines of evidence suggest that PPO responds to environmental conditions and could be involved in the response of plants to abiotic stress. This review highlights our current understanding of the in vivo functions of PPO and considers the potential opportunities it presents for exploitation to increase stress tolerance in food crops.

Abstract: Maize (Zea mays L) is one of the most important food crops and very common in all parts of sub-Saharan Africa. In 2010 53 million tons of maize were produced in sub-Saharan Africa on about one third of the total harvested cropland area (similar to 33 million ha). Our aim is to identify the limiting agroclimatic variable for maize growth and development in sub-Saharan Africa by analyzing the separated and combined effects of temperature and precipitation. Under changing climate, both climate variables are projected to change severely, and their impacts on crop yields are frequently assessed using process-based crop models. However it is often unclear which agroclimatic variable will have the strongest influence on crop growth and development under climate change and previous studies disagree over this question. We create synthetic climate data in order to study the effect of large changes in the length of the wet season and the amount of precipitation during the wet season both separately and in combination with changes in temperature. The dynamic global vegetation model for managed land LPJmL is used to simulate maize yields under current and future climatic conditions for the two 10-year periods 2056-2065 and 2081-2090 for three climate scenarios for the A1b emission scenario but without considering the beneficial CO2 fertilization effect. The importance of temperature and precipitation effects on maize yields varies spatially and we identify four groups of crop yield changes: regions with strong negative effects resulting from climate change (<-33% yield change), regions with moderate (-33% to -10% yield change) or slight negative effects (-10% to +6% yield change), and regions with positive effects arising from climate change mainly in currently temperature-limited high altitudes (>+6% yield change). In the first three groups temperature increases lead to maize yield reductions of 3 to 20%, with the exception of mountainous and thus cooler regions in South and East Africa. A reduction of the wet season precipitation causes decreases in maize yield of at least 30% and prevails over the effect of increased temperatures in southern parts of Mozambique and Zambia, the Sahel and parts of eastern Africa in the two projection periods. This knowledge about the limiting abiotic stress factor in each region will help to prioritize future research needs in modeling of agricultural systems as well as in drought and heat stress breeding programs and to identify adaption options in agricultural development projects. On the other hand the study enhances the understanding of temperature and water stress effects on crop yields in a global vegetation model in order to identify future research and model development needs. (C) 2013 Elsevier B.V. All rights reserved.