Abstract: Agricultural nitrous oxide (N2O) pollution resulting from the use of synthetic fertilizers represents a significant contribution to anthropogenic greenhouse gas emissions, providing a rationale for reduced use of nitrogen (N) fertilizers. Nitrogen limitation results in extensive systems rebalancing that remodels metabolism and defence processes. To analyse the regulation underpinning these responses, barley (Horedeum vulgare) seedlings were grown for 7 d under N-deficient conditions until net photosynthesis was 50% lower than in N-replete controls. Although shoot growth was decreased there was no evidence for the induction of oxidative stress despite lower total concentrations of N-containing antioxidants. Nitrogen-deficient barley leaves were rich in amino acids, sugars and tricarboxylic acid cycle intermediates. In contrast to N-replete leaves one-day-old nymphs of the green peach aphid (Myzus persicae) failed to reach adulthood when transferred to N-deficient barley leaves. Transcripts encoding cell, sugar and nutrient signalling, protein degradation and secondary metabolism were over-represented in N-deficient leaves while those associated with hormone metabolism were similar under both nutrient regimes with the exception of mRNAs encoding proteins involved in auxin metabolism and responses. Significant similarities were observed between the N-limited barley leaf transcriptome and that of aphid-infested Arabidopsis leaves. These findings not only highlight significant similarities between biotic and abiotic stress signalling cascades but also identify potential targets for increasing aphid resistance with implications for the development of sustainable agriculture.

Abstract: In the arable farming region Flevoland in The Netherlands climate change, including extreme events and pests and diseases, will likely pose risks to a variety of crops including high value crops such as seed potato, ware potato and seed onion. A well designed adaptation strategy at the farm level can reduce risks for farmers in Flevoland. Currently, most of the impact assessments rely heavily on (modelling) techniques that cannot take into account extreme events and pests and diseases and cannot address all crops, and are thus not suited as input for a comprehensive adaptation strategy at the farm level. To identify major climate risks and impacts and develop an adaptation measure portfolio for the most relevant risks we complemented crop growth modelling with a semi-quantitative and participatory approach, the Agro Climatic Calendar (ACC), A cost-benefit analysis and stakeholder workshops were used to identify robust adaptation measures and design an adaptation strategy for contrasting scenarios in 2050. For Flevoland, potential yields of main crops were projected to increase, but five main climate risks were identified, and these are likely to offset the positive impacts. Optimized adaptation strategies differ per scenario (frequency of occurrence of climate risks) and per farm (difference in economic loss). When impacts are high (in the +2 degrees C and A1 SRES scenario) drip irrigation was identified as the best adaptation measure against the main climate risk heat wave that causes second-growth in seed and ware potato. When impacts are smaller (the +1 degrees C and B2 SRES scenario), other options including no adaptation are more cost-effective. Our study shows that with relatively simple techniques such as the ACC combined with a stakeholder process, adaptation strategies can be designed for whole farming systems. Important benefits of this approach compared to modelling techniques are that all crops can be included, all climate factors can be addressed, and a large range of adaptation measures can be explored. This enhances that the identified adaptation strategies are recognizable and relevant for stakeholders. (C) 2013 Elsevier B.V. All rights reserved.

Abstract: Soils are a significant source for the secondary greenhouse gas NO and assumed to be a significant source of tropospheric NOx in rural areas. Here we tested the LandscapeDNDC model for its capability to simulate magnitudes and dynamics of soil NO emissions for 22 sites differing in land use (arable, grassland and forest) and edaphic as well as climatic conditions. Overall, LandscapeDNDC simulated mean soil NO emissions agreed well with observations (r(2) = 0.82). However, simulated day to day variations of NO did only agree weakly with high temporal resolution measurements, though agreement between simulations and measurements significantly increased if data were aggregated to weekly, monthly and seasonal time scales. The model reproduced NO emissions from high and low emitting sites, and responded to fertilization (mineral and organic) events with pulse emissions. After evaluation, we linked the LandscapeDNDC model to a GIS database holding spatially explicit data on climate, land use, soil and management to quantify the contribution of soil biogenic NO emissions to the total NOx budget for the State of Saxony, Germany. Our calculations show that soils of both agricultural and forest systems are significant sources and contribute to about 8% (uncertainty range: 6 -13%) to the total annual tropospheric NO, budget for Saxony. However, the contributions of soil NO emission to total tropospheric NO, showed a high spatial variability and in some rural regions such as the Ore Mts., simulated soil NO emissions were by far more important than anthropogenic sources. (C) 2016 Elsevier Ltd. All rights reserved.