Quain, M. D., Makgopa, M. E., Marquez-Garcia, B., Comadira, G., Fernandez-Garcia, N., Olmos, E., et al. (2014). Ectopic phytocystatin expression leads to enhanced drought stress tolerance in soybean (Glycine max) and Arabidopsis thaliana through effects on strigolactone pathways and can also result in improved seed traits. Plant Biotechnol. J., 12(7), 903–913.
Abstract: Ectopic cystatin expression has long been used in plant pest management, but the cysteine protease, targets of these inhibitors, might also have important functions in the control of plant lifespan and stress tolerance that remain poorly characterized. We therefore characterized the effects of expression of the rice cystatin, oryzacystatin-I (OCI), on the growth, development and stress tolerance of crop (soybean) and model (Arabidopsis thaliana) plants. Ectopic OCI expression in soybean enhanced shoot branching and leaf chlorophyll accumulation at later stages of vegetative development and enhanced seed protein contents and decreased the abundance of mRNAs encoding strigolactone synthesis enzymes. The OCI-expressing A. thaliana showed a slow-growth phenotype, with increased leaf numbers and enhanced shoot branching at flowering. The OCI-dependent inhibition of cysteine proteases enhanced drought tolerance in soybean and A. thaliana, photosynthetic CO2 assimilation being much less sensitive to drought-induced inhibition in the OCI-expressing soybean lines. Ectopic OCI expression or treatment with the cysteine protease inhibitor E64 increased lateral root densities in A. thaliana. E64 treatment also increased lateral root densities in the max2-1 mutants that are defective in strigolactone signalling, but not in the max3-9 mutants that are defective in strigolactone synthesis. Taken together, these data provide evidence that OCI-inhibited cysteine proteases participate in the control of growth and stress tolerance through effects on strigolactones. We conclude that cysteine proteases are important targets for manipulation of plant growth, development and stress tolerance, and also seed quality traits.
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Ben Touhami, H., Lardy, R., Klumpp, K., & Bellocchi, G. (2013). Bayesian calibration of the Pasture Simulation model (PaSim) to simulate emissions from long-term European grassland sites: a case study at Laqueuille (France)..
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Bellocchi, G., & Ehrhardt, F. (2014). Collaborations with initiatives and projects outside MACSUR and AgMIP – Grassland & Livestock..
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Stefańczyk, E., Sobkowiak, S., Brylińska, M., & Śliwka, J. (2016). Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland. Eur. J. Plant Pathol., .
Abstract: Fusarium spp. belong to the division Ascomycota and cause important plant diseases; these fungi may contaminate food products with mycotoxins, endangering human and animal health. Several Fusarium spp. have been associated with potato dry rot. The most frequent and devastating of these species are F. sambucinum, F. solani and F. oxysporum, depending on the geographic location and the season. Samples of potato tubers with dry rot symptoms were collected, and their putative fungal isolates were identified as Fusarium species using partial nucleotide sequences of the internal transcribed spacer, translation elongation factor 1-α and β-tubulin genes. Among 149 isolates, 12 species were identified. F. oxysporum was the most frequent (45 % of the isolates), followed by F. avenaceum (12.1 %), F. solani (10.7 %) and F. sambucinum (7.4 %). Phylogenetic analyses confirmed the species identifications and revealed a high diversity of F. solani and a low diversity of F. oxysporum. Potential producers of zearalenone and trichothecenes were identified within the obtained isolates using PCR markers. Isolates that were pathogenic to potatoes in laboratory tests were found in four species: F. sambucinum, F. avenaceum, F. culmorum, and F. graminearum. The effects of increased temperature and mixed inoculum on the pathogenicities of chosen species were evaluated. This study adds 434 potato-derived Fusarium sequences to the NCBI GenBank database and demonstrates that the list of Fusarium species and mycotoxins present in potato tubers may be richer than previously believed, regardless of whether these species cause dry rot or live as saprophytes.
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Seddaiu, G., Iocola, I., Farina, R., Orsini, R., Iezzi, G., & Roggero, P. P. (2016). Long term effects of tillage practices and N fertilization in rainfed Mediterranean cropping systems: durum wheat, sunflower and maize grain yield. European Journal of Agronomy, 77, 166–178.
Abstract: Long term investigations on the combined effects of tillage systems and other agronomic practices such as mineral N fertilization under Mediterranean conditions on durum wheat are very scanty and findings are often contradictory. Moreover, no studies are available on the long term effect of the adoption of conservation tillage on grain yield of maize and sunflower grown in rotation with durum wheat under rainfed Mediterranean conditions. This paper reports the results of a 20-years experiment on a durum wheat-sunflower (7 years) and durum wheat–maize (13 years) two-year rotation, whose main objective was to quantify the long term effects of different tillage practices (CT = conventional tillage; MT = minimum tillage; NT = no tillage) combined with different nitrogen fertilizer rates (N0, N1, N2 corresponding to 0, 45 and 90 kg N ha−1 for sunflower, and 0, 90 and 180 kg N ha−1 for wheat and maize) on grain yield, yield components and yield stability for the three crops. In addition, the influence of meteorological factors on the interannual variability of studied variables was also assessed. For durum wheat, NT did not allow substantial yield benefits leading to comparable yields with respect to CT in ten out of twenty years. For both sunflower and maize, NT under rainfed conditions was not a viable options, because of the unsuitable (i.e., too wet) soil conditions of the clayish soil at sowing. Both spring crops performed well with MT. No significant N × tillage interaction was found for the three crops. As expected, the response of durum wheat and maize grain yield to N was remarkable, while sunflower grain yield was not significantly influenced by N rate. Wheat yield was constrained by high temperatures in January during tillering and drought in April during heading. The interannual yield variability of sunflower was mainly associated to soil water deficit at flowering and air temperature during seed filling. Heavy rains during this latter phase strongly constrained sunflower grain yield. Maize grain yield was negatively affected by high temperatures in June and drought in July, this latter factor was particularly important in the fertilized maize. Considering both yield and yield stability, durum wheat and sunflower performed better under MT and N1 while maize performed better under both CT and MT and with N2 rates. The results of this long term study are suitable for supporting policies on sustainable Mediterranean rainfed cropping systems and also for cropping system modelling.
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