Abstract: Ectopic cystatin expression has long been used in plant pest management, but the cysteine protease, targets of these inhibitors, might also have important functions in the control of plant lifespan and stress tolerance that remain poorly characterized. We therefore characterized the effects of expression of the rice cystatin, oryzacystatin-I (OCI), on the growth, development and stress tolerance of crop (soybean) and model (Arabidopsis thaliana) plants. Ectopic OCI expression in soybean enhanced shoot branching and leaf chlorophyll accumulation at later stages of vegetative development and enhanced seed protein contents and decreased the abundance of mRNAs encoding strigolactone synthesis enzymes. The OCI-expressing A. thaliana showed a slow-growth phenotype, with increased leaf numbers and enhanced shoot branching at flowering. The OCI-dependent inhibition of cysteine proteases enhanced drought tolerance in soybean and A. thaliana, photosynthetic CO2 assimilation being much less sensitive to drought-induced inhibition in the OCI-expressing soybean lines. Ectopic OCI expression or treatment with the cysteine protease inhibitor E64 increased lateral root densities in A. thaliana. E64 treatment also increased lateral root densities in the max2-1 mutants that are defective in strigolactone signalling, but not in the max3-9 mutants that are defective in strigolactone synthesis. Taken together, these data provide evidence that OCI-inhibited cysteine proteases participate in the control of growth and stress tolerance through effects on strigolactones. We conclude that cysteine proteases are important targets for manipulation of plant growth, development and stress tolerance, and also seed quality traits.

Abstract: Climate change and socioeconomic developments will have a decisive impact on people exposed to hunger. This study analyses climate change impacts on agriculture and potential implications for the occurrence of hunger under different socioeconomic scenarios for 2030, focusing on the world regions most affected by poverty today: the Middle East and North Africa, South Asia, and Sub-Saharan Africa. We use a spatially explicit, agroeconomic land-use model to assess agricultural vulnerability to climate change. The aims of our study are to provide spatially explicit projections of climate change impacts on Costs of Food, and to combine them with spatially explicit hunger projections for the year 2030, both under a poverty, as well as a prosperity scenario. Our model results indicate that while average yields decrease with climate change in all focus regions, the impact on the Costs of Food is very diverse. Costs of Food increase most in the Middle East and North Africa, where available agricultural land is already fully utilized and options to import food are limited. The increase is least in Sub-Saharan Africa, since production there can be shifted to areas which are only marginally affected by climate change and imports from other regions increase. South Asia and Sub-Saharan Africa can partly adapt to climate change, in our model, by modifying trade and expanding agricultural land. In the Middle East and North Africa, almost the entire population is affected by increasing Costs of Food, but the share of people vulnerable to hunger is relatively low, due to relatively strong economic development in these projections. In Sub-Saharan Africa, the Vulnerability to Hunger will persist, but increases in Costs of Food are moderate. While in South Asia a high share of the population suffers from increases in Costs of Food and is exposed to hunger, only a negligible number of people will be exposed at extreme levels. Independent of the region, the impacts of climate change are less severe in a richer and more globalized world. Adverse climate impacts on the Costs of Food could be moderated by promoting technological progress in agriculture. Improving market access would be advantageous for farmers, providing the opportunity to profitably increase production in the Middle East and North Africa as well as in South Asia, but may lead to increasing Costs of Food for consumers. In the long-term perspective until 2080, the consequences of climate change will become even more severe: while in 2030 56% of the global population may face increasing Costs of Food in a poor and fragmented world, in 2080 the proportion will rise to 73%.

Abstract: Human food security requires the production of sufficient quantities of both high-quality protein and dietary energy. In a series of case-studies from New Zealand, we show that while production of food ingredients from crops on arable land can meet human dietary energy requirements effectively, requirements for high-quality protein are met more efficiently by animal production from such land. We present a model that can be used to assess dietary energy and quality-corrected protein production from various crop and crop/animal production systems, and demonstrate its utility. We extend our analysis with an accompanying economic analysis of commercially available pre-prepared or simply-cooked foods that can be produced from our case-study crop and animal products. We calculate the per-person, per-day cost of both quality-corrected protein and dietary energy as provided in the processed foods. We conclude that mixed dairy/cropping systems provide the greatest quantity of high quality protein per unit price to the consumer, have the highest food energy production and can support the dietary requirements of the highest number of people, when assessed as all-year-round production systems. Global food and nutritional security will largely be an outcome of national or regional agroeconomies addressing their town food needs. We hope that lour model will be used for similar analyses of food production systems in other countries, agroecological zones and economies.