Abstract: Phytocystatins are a well-characterized class of naturally occurring protease inhibitors that function by preventing the catalysis of papain-like cysteine proteases. The action of cystatins in biotic stress resistance has been studied intensively, but relatively little is known about their functions in plant growth and defence responses to abiotic stresses, such as drought. Extreme weather events, such as drought and flooding, will have negative impacts on the yields of crop plants, particularly grain legumes. The concepts that changes in cellular protein content and composition are required for acclimation to different abiotic stresses, and that these adjustments are achieved through regulation of proteolysis, are widely accepted. However, the nature and regulation of the protein turnover machinery that underpins essential stress-induced cellular restructuring remain poorly characterized. Cysteine proteases are intrinsic to the genetic programmes that underpin plant development and senescence, but their functions in stress-induced senescence are not well defined. Transgenic plants including soybean that have been engineered to constitutively express phytocystatins show enhanced tolerance to a range of different abiotic stresses including drought, suggesting that manipulation of cysteine protease activities by altered phytocystatin expression in crop plants might be used to improve resilience and quality in the face of climate change.

Abstract: Due to the more frequent use of crop models at regional and national scale, the effects of spatial data input resolution have gained increased attention. However, little is known about the influence of variability in crop management on model outputs. A constant and uniform crop management is often considered over the simulated area and period. This study determines the influence of crop management adapted to climatic conditions and input data resolution on regional-scale outputs of crop models. For this purpose, winter wheat and maize were simulated over 30 years with spatially and temporally uniform management or adaptive management for North Rhine-Westphalia ((similar to)34 083 km(2)), Germany. Adaptive management to local climatic conditions was used for 1) sowing date, 2) N fertilization dates, 3) N amounts, and 4) crop cycle length. Therefore, the models were applied with four different management sets for each crop. Input data for climate, soil and management were selected at five resolutions, from 1 x 1 km to 100 x 100 km grid size. Overall, 11 crop models were used to predict regional mean crop yield, actual evapotranspiration, and drainage. Adaptive management had little effect (< 10% difference) on the 30-year mean of the three output variables for most models and did not depend on soil, climate, and management resolution. Nevertheless, the effect was substantial for certain models, up to 31% on yield, 27% on evapotranspiration, and 12% on drainage compared to the uniform management reference. In general, effects were stronger on yield than on evapotranspiration and drainage, which had little sensitivity to changes in management. Scaling effects were generally lower than management effects on yield and evapotranspiration as opposed to drainage. Despite this trend, sensitivity to management and scaling varied greatly among the models. At the annual scale, effects were stronger in certain years, particularly the management effect on yield. These results imply that depending on the model, the representation of management should be carefully chosen, particularly when simulating yields and for predictions on annual scale.

Abstract: Justifiable usage of large-scale crop model simulations requires transparent, comprehensive and spatially extensive evaluations of their performance and associated accuracy. Simulated crop yields of a Pan-European implementation of the Environmental Policy Integrated Climate (EPIC) crop model were satisfactorily evaluated with reported regional yield data from EUROSTAT for four major crops, including winter wheat, rainfed and irrigated maize, spring barley and winter rye. European-wide land use, elevation, soil and daily meteorological gridded data were integrated in GIS and coupled with EPIC. Default EPIC crop and biophysical process parameter values were used with some minor adjustments according to suggestions from scientific literature. The model performance was improved by spatial calculations of crop sowing densities, potential heat units, operation schedules, and nutrient application rates. EPIC performed reasonable in the simulation of regional crop yields, with long-term averages predicted better than inter-annual variability: linear regression R-2 ranged from 0.58 (maize) to 0.91 (spring barley) and relative estimation errors were between +/- 30% for most of the European regions. The modelled and reported crop yields demonstrated similar responses to driving meteorological variables. However, EPIC performed better in dry compared to wet years. A yield sensitivity analysis of crop nutrient and irrigation management factors and cultivar specific characteristics for contrasting regions in Europe revealed a range in model response and attainable yields. We also show that modelled crop yield is strongly dependent on the chosen PET method. The simulated crop yield variability was lower compared to reported crop yields. This assessment should contribute to the availability of harmonised and transparently evaluated agricultural modelling tools in the EU as well as the establishment of modelling benchmarks as a requirement for sound and ongoing policy evaluations in the agricultural and environmental domains. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.