Abstract: This study evaluates the effect of planting three cover crops (CCs) (barley, Hordeum vulgare L.; vetch, Vicia villosa L.; rape, Brassica napus L.) on the direct emission of N(2)O, CO(2) and CH(4) in the intercrop period and the impact of incorporating these CCs on the emission of greenhouse gas (GHG) from the forthcoming irrigated maize (Zea mays L.) crop. Vetch and barley were the CCs with the highest N(2)O and CO(2) losses (75 and 47% increase compared with the control, respectively) in the fallow period. In all cases, fluxes of N(2)O were increased through N fertilization and the incorporation of barley and rape residues (40 and 17% increase, respectively). The combination of a high C:N ratio with the addition of an external source of mineral N increased the fluxes of N(2)O compared with -Ba and -Rp. The direct emissions of N(2)O were lower than expected for a fertilized crop (0.10% emission factor, EF) compared with other studies and the IPCC EF. These results are believed to be associated with a decreased NO(3)(-) pool due to highly denitrifying conditions and increased drainage. The fluxes of CO(2) were in the range of other fertilized crops (i.e., 1118.71-1736.52 kg CO(2)-Cha(-1)). The incorporation of CC residues enhanced soil respiration in the range of 21-28% for barley and rape although no significant differences between treatments were detected. Negative CH(4) fluxes were measured and displayed an overall sink effect for all incorporated CC (mean values of -0.12 and -0.10 kg CH(4)-Cha(-1) for plots with and without incorporated CCs, respectively).

Abstract: In this study, we compare the relative importance of climate change to technological, management, price and policy changes on European arable farming systems. This required linking four models: the SIMPLACE crop growth modelling framework to calculate future yields under climate change for arable crops; the CAPRI model to estimate impacts on global agricultural markets, specifically product prices; the bio-economic farm model FSSIM to calculate the future changes in cropping patterns and farm net income at the farm and regional level; and the environmental model INTEGRATOR to calculate nitrogen (N) uptake and losses to air and water. First, the four linked models were applied to analyse the effect of climate change only or a most likely baseline (i.e. B1) scenario for 2050 as well as for two alternative scenarios with, respectively, strong (i.e. A1-b1) and weak economic growth (B2) for five regions/countries across Europe (i.e. Denmark, Flevoland, Midi Pyrenees, Zachodniopomorsld and Andalucia). These analyses Were repeated but assuming in addition to climate change impacts, also the effects of changes in technology and management on crop yields, the effects of changes in prices and policies in 2050, and the effects of all factors together. The outcomes show that the effects of climate change to 2050 result in higher farm net incomes in the Northern and Northern-Central EU regions, in practically unchanged farm net incomes in the Central and Central-Southern EU regions, and in much lower farm net incomes in Southern EU regions compared to those in the base year. Climate change in combination with improved technology and farm management and/or with price changes towards 2050 results in a higher to much higher farm net incomes. Increases in farm net income for the B1 and A1-b1 scenarios are moderately stronger than those for the B2 scenario, due to the smaller increases in product prices and/or yields for the B2 scenario. Farm labour demand slightly to moderately increases towards 2050 as related to changes in cropping patterns. Changes in N2O emissions and N leaching compared to the base year are mainly caused by changes in total N inputs from the applied fertilizers and animal manure, which in turn are influenced by changes in crop yields and cropping patterns, whereas NH3 emissions are mainly determined by assumed improvements in manure application techniques. N emissions and N leaching strongly increase in Denmark and Zachodniopomorski, slightly decrease to moderately increase in Flevoland and Midi-Pyrenees, and strongly decrease in Andalucia, except for NH3 emissions which zero to moderately decrease in Flevoland and Denmark. (C) 2015 Elsevier Ltd. All tights reserved.

Abstract: Agricultural nitrous oxide (N2O) pollution resulting from the use of synthetic fertilizers represents a significant contribution to anthropogenic greenhouse gas emissions, providing a rationale for reduced use of nitrogen (N) fertilizers. Nitrogen limitation results in extensive systems rebalancing that remodels metabolism and defence processes. To analyse the regulation underpinning these responses, barley (Horedeum vulgare) seedlings were grown for 7 d under N-deficient conditions until net photosynthesis was 50% lower than in N-replete controls. Although shoot growth was decreased there was no evidence for the induction of oxidative stress despite lower total concentrations of N-containing antioxidants. Nitrogen-deficient barley leaves were rich in amino acids, sugars and tricarboxylic acid cycle intermediates. In contrast to N-replete leaves one-day-old nymphs of the green peach aphid (Myzus persicae) failed to reach adulthood when transferred to N-deficient barley leaves. Transcripts encoding cell, sugar and nutrient signalling, protein degradation and secondary metabolism were over-represented in N-deficient leaves while those associated with hormone metabolism were similar under both nutrient regimes with the exception of mRNAs encoding proteins involved in auxin metabolism and responses. Significant similarities were observed between the N-limited barley leaf transcriptome and that of aphid-infested Arabidopsis leaves. These findings not only highlight significant similarities between biotic and abiotic stress signalling cascades but also identify potential targets for increasing aphid resistance with implications for the development of sustainable agriculture.