Abstract: The frequency and intensity of extreme high temperature events are expected to increase with climate change. Higher temperatures near anthesis have a large negative effect on maize (Zea mays, L.) grain yield. While crop growth models are commonly used to assess climate change impacts on maize and other crops, it is only recently that they have accounted for such heat stress effects, despite limited field data availability for model evaluation. There is also increasing awareness but limited testing of the importance of canopy temperature as compared to air temperature for heat stress impact simulations. In this study, four independent irrigated field trials with controlled heating imposed using polyethylene shelters were used to develop and evaluate a heat stress response function in the crop modeling framework SIMPLACE, in which the Lintul5 crop model was combined with a canopy temperature model. A dataset from Argentina with the temperate hybrid Nidera AX 842 MG (RM 119) was used to develop a yield reduction function based on accumulated hourly stress thermal time above a critical temperature of 34 degrees C. A second dataset from Spain with a FAO 700 cultivar was used to evaluate the model with daily weather inputs in two sets of simulations. The first was used to calibrate SIMPLACE for conditions with no heat stress, and the second was used to evaluate SIMPLACE under conditions of heat stress using the reduction factor obtained with the Argentine dataset. Both sets of simulations were conducted twice; with the heat stress function alternatively driven with air and simulated canopy temperature. Grain yield simulated under heat stress conditions improved when canopy temperature was used instead of air temperature (RMSE equal to 175 and 309 g m(-2), respectively). For the irrigated and high radiative conditions, raising the critical threshold temperature for heat stress to 39 degrees C improved yield simulation using air temperature (RMSE: 221 gm(-2)) without the need to simulate canopy temperature (RMSE: 175 gm(-2)). However, this approach of adjusting thresholds is only likely to work in environments where climatic variables and the level of soil water deficit are constant, such as irrigated conditions and are not appropriate for rainfed production conditions. (C) 2016 Elsevier B.V. All rights reserved.

Abstract: Fusarium spp. belong to the division Ascomycota and cause important plant diseases; these fungi may contaminate food products with mycotoxins, endangering human and animal health. Several Fusarium spp. have been associated with potato dry rot. The most frequent and devastating of these species are F. sambucinum, F. solani and F. oxysporum, depending on the geographic location and the season. Samples of potato tubers with dry rot symptoms were collected, and their putative fungal isolates were identified as Fusarium species using partial nucleotide sequences of the internal transcribed spacer, translation elongation factor 1-α and β-tubulin genes. Among 149 isolates, 12 species were identified. F. oxysporum was the most frequent (45 % of the isolates), followed by F. avenaceum (12.1 %), F. solani (10.7 %) and F. sambucinum (7.4 %). Phylogenetic analyses confirmed the species identifications and revealed a high diversity of F. solani and a low diversity of F. oxysporum. Potential producers of zearalenone and trichothecenes were identified within the obtained isolates using PCR markers. Isolates that were pathogenic to potatoes in laboratory tests were found in four species: F. sambucinum, F. avenaceum, F. culmorum, and F. graminearum. The effects of increased temperature and mixed inoculum on the pathogenicities of chosen species were evaluated. This study adds 434 potato-derived Fusarium sequences to the NCBI GenBank database and demonstrates that the list of Fusarium species and mycotoxins present in potato tubers may be richer than previously believed, regardless of whether these species cause dry rot or live as saprophytes.

Abstract: The DNDC, LandscapeDNDC and IAP-N-GAS models have been designed to simulate the carbon and nitrogen processes of terrestrial ecosystems. Until now, a comparison of these models using simultaneous observations has not been reported, although such a comparison is essential for further model development and application. This study aimed to evaluate the performance of the models, delineate the strengths and limitations of each model for simulating soil nitrous oxide (N2O) and nitric oxide (NO) emissions, and explore short-comings of these models that may require reconsideration. We conducted comparisons among the models using simultaneous observations of both gases and relevant variables from the winter wheat-summer maize rotation system at three field sites with calcareous soils. Simulations of N2O and NO emissions by the three models agreed well with annual observations, but not with daily observations. All models failed to correctly simulate soil moisture, which could explain some of the incorrect daily fluxes of N2O and NO, especially for intensive fluxes during the growing season. Multi-model ensembles are promising approaches to better simulate daily gas emissions. IAP-N-GAS underestimated the priming effect of straw incorporation on N2O and NO emissions, but better results were obtained with DNDC95 and LandscapeDNDC. LandscapeDNDC and IAP-N-GAS need to improve the simulation of irrigation water allocation and residue decomposition processes, respectively, and together to distinguish different irrigation methods as DNDC95 does. All three models overestimated the emissions of the nitrogenous gases for high nitrogen fertilizer (>430 kg N ha(-1) yr(-1)) addition treatments, and therefore, future research should focus more on the simulation of the limitation of soil dissolvable organic carbon on denitrification in calcareous soils.