Abstract: Two independently developed simulation models – the grassland-specific PaSim and the biome-generic Biome-BGC MuSo (BBGC MuSo) – linking climate, soil, vegetation and management to ecosystem biogeochemical cycles were compared in a simulation of carbon (C) and water fluxes. The results were assessed against eddy-covariance flux data from five observational grassland sites representing a range of conditions in Europe: Grillenburg in Germany, Laqueuille in France with both extensive and intensive management, Monte Bondone in Italy and Oensingen in Switzerland. Model comparison (after calibration) gave substantial agreement, the performances being marginal to acceptable for weekly-aggregated gross primary production and ecosystem respiration (R-2 similar to 0.66 – 0.91), weekly evapotranspiration (R-2 similar to 0.78 – 0.94), soil water content in the topsoil (R-2 similar to 0.1 -0.7) and soil temperature (R-2 similar to 0.88 – 0.96). The bias was limited to the range -13 to 9 g C m(-2) week(-1) for C fluxes (-11 to 8 g C m(-2) week(-1) in case of BBGC MuSo, and -13 to 9 g C m(-2) week(-1) in case of PaSim) and -4 to 6 mm week for water fluxes (with BBGC MuSo providing somewhat higher estimates than PaSim), but some higher relative root mean square errors indicate low accuracy for prediction, especially for net ecosystem exchange The sensitivity of simulated outputs to changes in atmospheric carbon dioxide concentration ([CO2]), temperature and precipitation indicate, with certain agreement between the two models, that C outcomes are dominated by [CO2] and temperature gradients, and are less due to precipitation. ET rates decrease with increasing [CO2] in PaSim (consistent with experimental knowledge), while lack of appropriate stomatal response could be a limit in BBGC MuSo responsiveness. Results of the study indicate that some of the errors might be related to the improper representation of soil water content and soil temperature. Improvement is needed in the model representations of soil processes (especially soil water balance) that strongly influence the biogeochemical cycles of managed and unmanaged grasslands. (C) 2015 Elsevier B.V. All rights reserved.

Abstract: Polyphenol oxidase (PPO) catalyses the oxidation of monophenols and/or o-diphenols to o-quinones with the concomitant reduction of oxygen to water which results in protein complexing and the formation of brown melanin pigments. The most frequently suggested role for PPO in plants has been in defence against herbivores and pathogens, based on the physical separation of the chloroplast-localized enzyme from the vacuole-localized substrates. The o-quinone-protein complexes, formed as a consequence of cell damage, may reduce the nutritional value of the tissue and thereby reduce predation but can also participate in the formation of structural barriers against invading pathogens. However, since a sufficient level of compartmentation-based regulation could be accomplished if PPO was targeted to the cytosol, the benefit derived by some plant species in having PPO present in the chloroplast lumen remains an intriguing question. So is there more to the chloroplastic location of PPO? An interaction between PPO activity and photosynthesis has been proposed on more than one occasion but, to date, evidence either for or against direct involvement has been equivocal, and the lack of identified chloroplastic substrates remains an issue. Similarly, PPO has been suggested to have both pro- and anti-oxidant functions. Nevertheless, several independent lines of evidence suggest that PPO responds to environmental conditions and could be involved in the response of plants to abiotic stress. This review highlights our current understanding of the in vivo functions of PPO and considers the potential opportunities it presents for exploitation to increase stress tolerance in food crops.