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Boeckx, T., Winters, A. L., Webb, K. J., & Kingston-Smith, A. H. (2015). Polyphenol oxidase in leaves: is there any significance to the chloroplastic localization. J. Experim. Bot., 66(12), 3571–3579.
Abstract: Polyphenol oxidase (PPO) catalyses the oxidation of monophenols and/or o-diphenols to o-quinones with the concomitant reduction of oxygen to water which results in protein complexing and the formation of brown melanin pigments. The most frequently suggested role for PPO in plants has been in defence against herbivores and pathogens, based on the physical separation of the chloroplast-localized enzyme from the vacuole-localized substrates. The o-quinone-protein complexes, formed as a consequence of cell damage, may reduce the nutritional value of the tissue and thereby reduce predation but can also participate in the formation of structural barriers against invading pathogens. However, since a sufficient level of compartmentation-based regulation could be accomplished if PPO was targeted to the cytosol, the benefit derived by some plant species in having PPO present in the chloroplast lumen remains an intriguing question. So is there more to the chloroplastic location of PPO? An interaction between PPO activity and photosynthesis has been proposed on more than one occasion but, to date, evidence either for or against direct involvement has been equivocal, and the lack of identified chloroplastic substrates remains an issue. Similarly, PPO has been suggested to have both pro- and anti-oxidant functions. Nevertheless, several independent lines of evidence suggest that PPO responds to environmental conditions and could be involved in the response of plants to abiotic stress. This review highlights our current understanding of the in vivo functions of PPO and considers the potential opportunities it presents for exploitation to increase stress tolerance in food crops.
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Van Oijen, M., & Höglind, M. (2016). Toward a Bayesian procedure for using process-based models in plant breeding, with application to ideotype design. Euphytica, 207(3), 627–643.
Abstract: Process-based grassland models (PBMs) simulate growth and development of vegetation over time. The models tend to have a large number of parameters that represent properties of the plants. To simulate different cultivars of the same species, different parameter values are required. Parameter differences may be interpreted as genetic variation for plant traits. Despite this natural connection between PBMs and plant genetics, there are only few examples of successful use of PBMs in plant breeding. Here we present a new procedure by which PBMs can help design ideotypes, i.e. virtual cultivars that optimally combine properties of existing cultivars. Ideotypes constitute selection targets for breeding. The procedure consists of four steps: (1) Bayesian calibration of model parameters using data from cultivar trials, (2) Estimating genetic variation for parameters from the combination of cultivar-specific calibrated parameter distributions, (3) Identifying parameter combinations that meet breeding objectives, (4) Translating model results to practice, i.e. interpreting parameters in terms of practical selection criteria. We show an application of the procedure to timothy (Phleum pratense L.) as grown in different regions of Norway.
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Francone, C., Cassardo, C., Richiardone, R., & Confalonieri, R. (2012). Sensitivity Analysis and Investigation of the Behaviour of the UTOPIA Land-Surface Process Model: A Case Study for Vineyards in Northern Italy. Boundary-Layer Meteorology, 144(3), 419–430.
Abstract: We used sensitivity-analysis techniques to investigate the behaviour of the land-surface model UTOPIA while simulating the micrometeorology of a typical northern Italy vineyard (Vitis vinifera L.) under average climatic conditions. Sensitivity-analysis experiments were performed by sampling the vegetation parameter hyperspace using the Morris method and quantifying the parameter relevance across a wide range of soil conditions. This method was used since it proved its suitability for models with high computational time or with a large number of parameters, in a variety of studies performed on different types of biophysical models. The impact of input variability was estimated on reference model variables selected among energy (e.g. net radiation, sensible and latent heat fluxes) and hydrological (e.g. soilmoisture, surface runoff, drainage) budget components. Maximum vegetation cover and maximum leaf area index were ranked as the most relevant parameters, with sensitivity indices exceeding the remaining parameters by about one order of magnitude. Soil variability had a high impact on the relevance of most of the vegetation parameters: coefficients of variation calculated on the sensitivity indices estimated for the different soils often exceeded 100 %. The only exceptions were represented by maximum vegetation cover and maximum leaf area index, which showed a low variability in sensitivity indices while changing soil type, and confirmed their key role in affecting model results.
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Christen, B., Kjeldsen, C., Dalgaard, T., & Martin-Ortega, J. (2015). Can fuzzy cognitive mapping help in agricultural policy design and communication? Land Use Policy, 45, 64–75.
Abstract: Highlights •Fuzzy cognitive mapping (FCM)can help to improve agricultural policy design. •We analyse the views on regulation between farmers and non-farmers. •We demonstrate the utility of FCM in disentangling reasons for non-compliance. •Non-compliance is a result of dis-alignment of views rather than unwillingness. •FCM offers a critical, reflexive approach to how a regulatory process is conceived. Agricultural environmental regulation often fails to deliver the desired effects because of farmers adopting the related measures incorrectly or not at all. This is due to several barriers to the uptake of the prescribed environmentally beneficial farm management practices, most of which have been well established by social science research. Yet it is unclear why these barriers remain so difficult to overcome despite numerous and persistent attempts at the design, communication and enforcement of related agricultural policies. This paper examines the potential of fuzzy cognitive mapping (FCM) as a tool to disentangle the underlying reasons of this persistent problem. We present the FCM methodology as adapted to the application in a Scottish case study on how environmental regulation affects farmers and farming practice and what factors are important for compliance or non-compliance with this regulation. The study compares the views of two different stakeholder groups on this matter using FCM network visualizations that were validated by interviews and a workshop session. There was a farmers group representing a typical mix of Scottish farming systems and a non-farmers group, the latter comprising professionals from the fields of design, implementation, administration, consulting on and enforcement of agricultural policies. Between the two groups, the FCM process reveals a very different perception of importance and interaction of factors and strongly suggests that the problem lies in an institutional failure rather than in a simple unwillingness of farmers to obey the rules. FCM allows for a structured process of identifying areas of conflicting perceptions, but also areas where strongly differing groups of stakeholders might be able to gain common ground. In this way, FCM can help to identify anchoring points for targeted policy development and has the potential of becoming a useful tool in agricultural policy design and communication. Our results show the utility of FCM by pointing out how Scottish environmental regulation could be altered to increase compliance with the rules and where the reasons for the identified institutional failure might be sought.
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