Abstract: Fusarium spp. belong to the division Ascomycota and cause important plant diseases; these fungi may contaminate food products with mycotoxins, endangering human and animal health. Several Fusarium spp. have been associated with potato dry rot. The most frequent and devastating of these species are F. sambucinum, F. solani and F. oxysporum, depending on the geographic location and the season. Samples of potato tubers with dry rot symptoms were collected, and their putative fungal isolates were identified as Fusarium species using partial nucleotide sequences of the internal transcribed spacer, translation elongation factor 1-α and β-tubulin genes. Among 149 isolates, 12 species were identified. F. oxysporum was the most frequent (45 % of the isolates), followed by F. avenaceum (12.1 %), F. solani (10.7 %) and F. sambucinum (7.4 %). Phylogenetic analyses confirmed the species identifications and revealed a high diversity of F. solani and a low diversity of F. oxysporum. Potential producers of zearalenone and trichothecenes were identified within the obtained isolates using PCR markers. Isolates that were pathogenic to potatoes in laboratory tests were found in four species: F. sambucinum, F. avenaceum, F. culmorum, and F. graminearum. The effects of increased temperature and mixed inoculum on the pathogenicities of chosen species were evaluated. This study adds 434 potato-derived Fusarium sequences to the NCBI GenBank database and demonstrates that the list of Fusarium species and mycotoxins present in potato tubers may be richer than previously believed, regardless of whether these species cause dry rot or live as saprophytes.

Abstract: Field-scale crop models are often applied at spatial resolutions coarser than that of the arable field. However, little is known about the response of the models to spatially aggregated climate input data and why these responses can differ across models. Depending on the model, regional yield estimates from large-scale simulations may be biased, compared to simulations with high-resolution input data. We evaluated this so-called aggregation effect for 13 crop models for the region of North Rhine-Westphalia in Germany. The models were supplied with climate data of 1 km resolution and spatial aggregates of up to 100 km resolution raster. The models were used with 2 crops (winter wheat and silage maize) and 3 production situations (potential, water-limited and nitrogen-water-limited growth) to improve the understanding of errors in model simulations related to data aggregation and possible interactions with the model structure. The most important climate variables identified in determining the model-specific input data aggregation on simulated yields were mainly related to changes in radiation (wheat) and temperature (maize). Additionally, aggregation effects were systematic, regardless of the extent of the effect. Climate input data aggregation changed the mean simulated regional yield by up to 0.2 t ha(-1), whereas simulated yields from single years and models differed considerably, depending on the data aggregation. This implies that large-scale crop yield simulations are robust against climate data aggregation. However, large-scale simulations can be systematically biased when being evaluated at higher temporal or spatial resolution depending on the model and its parameterization.

Abstract: For spatial crop and agro-systems modelling, there is often a discrepancy between the scale of measured driving data and the target resolution. Spatial data aggregation is often necessary, which can introduce additional uncertainty into the simulation results. Previous studies have shown that climate data aggregation has little effect on simulation of phenological stages, but effects on net primary production (NPP) might still be expected through changing the length of the growing season and the period of grain filling. This study investigates the impact of spatial climate data aggregation on NPP simulation results, applying eleven different models for the same study region (∼34,000 km2), situated in Western Germany. To isolate effects of climate, soil data and management were assumed to be constant over the entire study area and over the entire study period of 29 years. Two crops, winter wheat and silage maize, were tested as monocultures. Compared to the impact of climate data aggregation on yield, the effect on NPP is in a similar range, but is slightly lower, with only small impacts on averages over the entire simulation period and study region. Maximum differences between the five scales in the range of 1–100 km grid cells show changes of 0.4–7.8% and 0.0–4.8% for wheat and maize, respectively, whereas the simulated potential NPP averages of the models show a wide range (1.9–4.2 g C m−2 d−1 and 2.7–6.1 g C m−2 d−1for wheat and maize, respectively). The impact of the spatial aggregation was also tested for shorter time periods, to see if impacts over shorter periods attenuate over longer periods. The results show larger impacts for single years (up to 9.4% for wheat and up to 13.6% for maize). An analysis of extreme weather conditions shows an aggregation effect in vulnerability up to 12.8% and 15.5% between the different resolutions for wheat and maize, respectively. Simulations of NPP averages over larger areas (e.g. regional scale) and longer time periods (several years) are relatively insensitive to climate data.