Abstract: Phytocystatins are a well-characterized class of naturally occurring protease inhibitors that function by preventing the catalysis of papain-like cysteine proteases. The action of cystatins in biotic stress resistance has been studied intensively, but relatively little is known about their functions in plant growth and defence responses to abiotic stresses, such as drought. Extreme weather events, such as drought and flooding, will have negative impacts on the yields of crop plants, particularly grain legumes. The concepts that changes in cellular protein content and composition are required for acclimation to different abiotic stresses, and that these adjustments are achieved through regulation of proteolysis, are widely accepted. However, the nature and regulation of the protein turnover machinery that underpins essential stress-induced cellular restructuring remain poorly characterized. Cysteine proteases are intrinsic to the genetic programmes that underpin plant development and senescence, but their functions in stress-induced senescence are not well defined. Transgenic plants including soybean that have been engineered to constitutively express phytocystatins show enhanced tolerance to a range of different abiotic stresses including drought, suggesting that manipulation of cysteine protease activities by altered phytocystatin expression in crop plants might be used to improve resilience and quality in the face of climate change.

Abstract: To deliver food security for the 9 billon population in 2050, a 70% increase in world food supply will be required. Projected climatic and environmental changes emphasize the need for breeding strategies that delivers both a substantial increase in yield potential and resilience to extreme weather events such as heat waves, late frost, and drought. Heat stress around sensitive stages of wheat development has been identified as a possible threat to wheat production in Europe. However, no estimates have been made to assess yield losses due to increased frequency and magnitude of heat stress under climate change. Using existing experimental data, the Sirius wheat model was refined by incorporating the effects of extreme temperature during flowering and grain filling on accelerated leaf senescence, grain number, and grain weight. This allowed us, for the first time, to quantify yield losses resulting from heat stress under climate change. The model was used to optimize wheat ideotypes for CMIP5-based climate scenarios for 2050 at six sites in Europe with diverse climates. The yield potential for heat-tolerant ideotypes can be substantially increased in the future (e.g. by 80% at Seville, 100% at Debrecen) compared with the current cultivars by selecting an optimal combination of wheat traits, e.g. optimal phenology and extended duration of grain filling. However, at two sites, Seville and Debrecen, the grain yields of heat-sensitive ideotypes were substantially lower (by 54% and 16%) and more variable compared with heat-tolerant ideotypes, because the extended grain filling required for the increased yield potential was in conflict with episodes of high temperature during flowering and grain filling. Despite much earlier flowering at these sites, the risk of heat stress affecting yields of heat-sensitive ideotypes remained high. Therefore, heat tolerance in wheat is likely to become a key trait for increased yield potential and yield stability in southern Europe in the future.

Abstract: Maize phenology observations at 112 national agro-meteorological experiment stations across China spanning the years 1981-2009 were used to investigate the spatiotemporal changes of maize phenology, as well as the relations to temperature change and cultivar shift. The greater scope of the dataset allows us to estimate the effects of temperature change and cultivar shift on maize phenology more precisely. We found that maize sowing date advanced significantly at 26.0% of stations mainly for spring maize in northwestern, southwestern and northeastern China, although delayed significantly at 8.0% of stations mainly in northeastern China and the North China Plain (NCP). Maize maturity date delayed significantly at 36.6% of stations mainly in the northeastern China and the NCP. As a result, duration of maize whole growing period (GPw) was prolonged significantly at 41.1% of stations, although mean temperature (Tmean) during GPw increased at 72.3% of stations, significantly at 19.6% of stations, and Tmean was negatively correlated with the duration of GPw at 92.9% of stations and significantly at 42.9% of stations. Once disentangling the effects of temperature change and cultivar shift with an approach based on accumulated thermal development unit, we found that increase in temperature advanced heading date and maturity date and reduced the duration of GPw at 81.3%, 82.1% and 83.9% of stations on average by 3.2, 6.0 and 3.5 days/decade, respectively. By contrast, cultivar shift delayed heading date and maturity date and prolonged the duration of GPw at 75.0%, 94.6% and 92.9% of stations on average by 1.5, 6.5 and 6.5 days/decade, respectively. Our results suggest that maize production is adapting to ongoing climate change by shift of sowing date and adoption of cultivars with longer growing period. The spatiotemporal changes of maize phenology presented here can further guide the development of adaptation options for maize production in near future.