Abstract: The accurate estimation of crop grain nitrogen (N; N in grain yield) is crucial for optimizing agricultural N management, especially in crop rotations. In the present study, 12 process-based models were applied to simulate the grain N of i) seven crops in rotations, ii) across various pedo-climatic and agro-management conditions in Europe, under both continuous simulation and single year simulation, and for iv) two calibration levels, namely minimal and detailed calibration. Generally, the results showed that the accuracy of the simulations in predicting grain N increased under detailed calibration. The models performed better in predicting the grain N of winter wheat (Triticum aestivum L.), winter barley (Hordewn vulgare L.) and spring barley (Hordeum vulgare L.) compared to spring oat (Avena saliva L.), winter rye (Secale cereale L.), pea (Piswn sativum L.) and winter oilseed rape (Brassica napus L.). These differences are linked to the intensity of parameterization with better parameterized crops showing lower prediction errors. The model performance was influenced by N fertilization and irrigation treatments, and a majority of the predictions were more accurate under low N and rainfed treatments. Moreover, the multi-model mean provided better predictions of grain N compared to any individual model. In regard to the Individual models, DAISY, FASSET, HERMES, MONICA and STICS are suitable for predicting grain N of the main crops in typical European crop rotations, which all performed well in both continuous simulation and single year simulation. Our results show that both the model initialization and the cover crop effects in crop rotations should be considered in order to achieve good performance of continuous simulation. Furthermore, the choice of either continuous simulation or single year simulation should be guided by the simulation objectives (e.g. grain yield, grain N content or N dynamics), the crop sequence (inclusion of legumes) and treatments (rate and type of N fertilizer) included in crop rotations and the model formalism.

Abstract: Highlights • The parameters to be fixed were consistent across sites. • Model calibration must be performed separately for each specific case. • Possible to reduce model parameters from 66 to 45. • Strong model reductions must be avoided. • The error term for the training data were characterised by timing (phase shift). Abstract Proper parameterisation and quantification of model uncertainty are two essential tasks in improvement and assessment of model performance. Bayesian calibration is a method that combines both tasks by quantifying probability distributions for model parameters and outputs. However, the method is rarely applied to complex models because of its high computational demand when used with high-dimensional parameter spaces. We therefore combined Bayesian calibration with sensitivity analysis, using the screening method by Morris (1991), in order to reduce model complexity by fixing parameters to which model output was only weakly sensitive to a nominal value. Further, the robustness of the model with respect to reduction in the number of free parameters were examined according to model discrepancy and output uncertainty. The process-based grassland model BASGRA was examined in the present study on two sites in Norway and in Germany, for two grass species (Phleum pratense and Arrhenatherum elatius). According to this study, a reduction of free model parameters from 66 to 45 was possible. The sensitivity analysis showed that the parameters to be fixed were consistent across sites (which differed in climate and soil conditions), while model calibration had to be performed separately for each combination of site and species. The output uncertainty decreased slightly, but still covered the field observations of aboveground biomass. Considering the training data, the mean square error for both the 66 and the 45 parameter model was dominated by errors in timing (phase shift), whereas no general pattern was found in errors when using the validation data. Stronger model reduction should be avoided, as the error term increased and output uncertainty was underestimated.