Abstract: Seeds are important to agriculture and conservation of plant biodiversity. In agriculture, seed germination performance is an important determinant of crop yield, in particular under adverse climatic conditions. Deterioration in seed quality is associated with the accumulation of cellular damage to macromolecules including lipids, protein, and DNA. Mechanisms that mitigate the deleterious cellular damage incurred in the quiescent state and in cycles of desiccation-hydration are crucial for the maintenance of seed viability and germination vigour. In early-imbibing seeds, damage to the embryo genome must be repaired prior to initiation of cell division to minimize growth inhibition and mutation of genetic information. Here we review recent advances that have established molecular links between genome integrity and seed quality. These studies identified that maintenance of genome integrity is particularly important to the seed stage of the plant lifecycle, revealing new insight into the physiological roles of plant DNA repair and recombination mechanisms. The high conservation of DNA repair and recombination factors across plant species underlines their potential as promising targets for the improvement of crop performance and development of molecular markers for prediction of seed vigour.

Abstract: In order to feed the world we need innovative thinking on how to increase agricultural production whilst also mitigating climate change. Agriculture and land-use change are responsible for approximately one-third of total anthropogenic greenhouse gas (GHG) emissions but hold potential for climate change mitigation but are only tangentially included in UNFCCC mitigation policies. To get a full estimate of GHG emissions from agricultural crop production both energy-based emissions and land-based emissions need to be accounted for. Furthermore, the major mitigation potential is likely to be indirect reduction of emissions i.e. reducing emissions per unit of agricultural product rather than the absolute emissions per se. Hence the system productivity must be included in the same analysis. This paper presents the Kaya-Porter identity, derived from the Maya identity, as a new way to calculate GHG emissions from agricultural crop production by deconstructing emissions into five elements; the GHG intensity of the energy used for production (kg CO2-eq./MJ), energy intensity of the production (MJ/kg dry matter), areal productivity (kg dry matter/ha), areal land-based GHG emissions (CO2-eq./ha) and area (ha). These separate elements in the identity can be targeted in emissions reduction and mitigation policies and are useful to analyse past and current trends in emissions and to explore future scenarios. Using the Kaya-Porter identity we have performed a case study on Danish crop production and find emissions to have been reduced by 12% from 1992 to 2008, whilst yields per unit area have remained constant. Both land-based emissions and energy-based emissions have decreased, mainly due to a 41% reduction in nitrogen fertilizer use. The initial identity based analysis for crop production presented here needs to be extended to include livestock to reflect the entire agricultural production and food demand sectors, thereby permitting analysis of the trade-offs between animal and plant food production, human dietary preferences and population and resulting GHG emissions. (C) 2012 Elsevier B.V. All rights reserved.

Abstract: The effects of short-and long-term salinisation were studied by comparing tomato growth on a soil exposed to one-season salinisation (short term) vs growth on a soil exposed to >20 years salinisation (long term). Remarkable differences were associated to substantial modifications of the soil physical-chemical characteristics in the root zone, including deteriorated structure, reduced infiltration properties and increased pH. Fresh yield, fruit number and fruit weight were similarly affected by short-and long-term salinisation. In contrast, the marketable yield was significantly lower in the long-term salinised soil-a response that was also associated to nutritional imbalance (mainly referred to P and K). As reported for plants growing under oxygen deprivation stress, the antioxidant capacity of the water soluble fraction of salinised tomato fruits was enhanced by short-term salinisation, also. Overall, long-term salinisation may cause physiological imbalances and yield reductions that cannot be solely attributed to hyperosmotic stress and ionic toxicity. Therefore, the ability of plants to cope with nutritional deficiency and withstand high pH and anoxia may be important traits that should be considered to improve plant tolerance to long-term salinised soils.