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Van Oosten, M. J., Sharkhuu, A., Batelli, G., Bressan, R. A., & Maggio, A. (2013). The Arabidopsis thaliana mutant air1 implicates SOS3 in the regulation of anthocyanins under salt stress. Plant Mol. Biol., 83(4-5), 405–415.
Abstract: The accumulation of anthocyanins in plants exposed to salt stress has been largely documented. However, the functional link and regulatory components underlying the biosynthesis of these molecules during exposure to stress are largely unknown. In a screen of second site suppressors of the salt overly sensitive3-1 (sos3-1) mutant, we isolated the anthocyanin-impaired-response-1 (air1) mutant. air1 is unable to accumulate anthocyanins under salt stress, a key phenotype of sos3-1 under high NaCl levels (120 mM). The air1 mutant showed a defect in anthocyanin production in response to salt stress but not to other stresses such as high light, low phosphorous, high temperature or drought stress. This specificity indicated that air1 mutation did not affect anthocyanin biosynthesis but rather its regulation in response to salt stress. Analysis of this mutant revealed a T-DNA insertion at the first exon of an Arabidopsis thaliana gene encoding for a basic region-leucine zipper transcription factor. air1 mutants displayed higher survival rates compared to wild-type in oxidative stress conditions, and presented an altered expression of anthocyanin biosynthetic genes such as F3H, F3’H and LDOX in salt stress conditions. The results presented here indicate that AIR1 is involved in the regulation of various steps of the flavonoid and anthocyanin accumulation pathways and is itself regulated by the salt-stress response signalling machinery. The discovery and characterization of AIR1 opens avenues to dissect the connections between abiotic stress and accumulation of antioxidants in the form of flavonoids and anthocyanins.
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Van Oijen, M., & Höglind, M. (2016). Toward a Bayesian procedure for using process-based models in plant breeding, with application to ideotype design. Euphytica, 207(3), 627–643.
Abstract: Process-based grassland models (PBMs) simulate growth and development of vegetation over time. The models tend to have a large number of parameters that represent properties of the plants. To simulate different cultivars of the same species, different parameter values are required. Parameter differences may be interpreted as genetic variation for plant traits. Despite this natural connection between PBMs and plant genetics, there are only few examples of successful use of PBMs in plant breeding. Here we present a new procedure by which PBMs can help design ideotypes, i.e. virtual cultivars that optimally combine properties of existing cultivars. Ideotypes constitute selection targets for breeding. The procedure consists of four steps: (1) Bayesian calibration of model parameters using data from cultivar trials, (2) Estimating genetic variation for parameters from the combination of cultivar-specific calibrated parameter distributions, (3) Identifying parameter combinations that meet breeding objectives, (4) Translating model results to practice, i.e. interpreting parameters in terms of practical selection criteria. We show an application of the procedure to timothy (Phleum pratense L.) as grown in different regions of Norway.
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Van Oijen, M., Cameron, D., Levy, P. E., & Preston, R. (2017). Correcting errors from spatial upscaling of nonlinear greenhouse gas flux models. Environmental Modelling & Software, 94, 157–165.
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Van Oijen, M. (2015). Methods for risk analysis and spatial upscaling of process-based models: Experiences from projects Carbo-Extreme and GREENHOUSE (Vol. 5).
Abstract: In the recently finished EU-funded project Carbo-Extreme, we developed a simple probabilistic method for quantifying vulnerabilities and risks to ecosystems (http://iopscience.iop.org/1748-9326/8/1/015032). The method defines risk as expected loss due to environmental hazards, and shows how such risk can be calculated as the product of ecosystem vulnerability and hazard probability. The method was used with six different vegetation models to estimate current and future drought risks for crops, grasslands and forests across Europe (http://www.biogeosciences.net/11/6357/2014/bg-11-6357-2014.html).In the still ongoing UK-funded project GREENHOUSE, the focus is on spatial upscaling of local measurements and model predictions of greenhouse gas emissions to wider regions. As part of this work, we are comparing different model upscaling methods – ranging from naive input aggregation to geostatistics – and quantify the uncertainties associated with the upscaling. This work builds on an earlier inventory of model upscaling methods that was produced in a collaboration of CEH-Edinburgh and the University of Bonn (https://www.stat.aau.at/Tagungen/statgis/2009/StatGIS2009Van%20Oijen1.pdf). Here we show a comparison of the methods using model predictions for the border region of England and Scotland. No Label
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van Middelkoop, J. C., & Kipling, R. P. (2017). Modelling the impact of climate change on livestock productivity at the farm-scale: An inventory of LiveM outcomes (Vol. 10).
Abstract: The report presented here provides an inventory of reports and conference papers produced by the partners of the livestock and grassland modelling theme (LiveM) of the Modelling European Agriculture with Climate Change for Food Security (MACSUR) knowledge hub. The findings presented illustrate the diverse nature of the multidisciplinary LiveM research community, and provide a reference source for those seeking to identify and pull out farm-level modelling outputs from the work of MACSUR and its partners. The survey of farm-scale outputs from LiveM revealed the interdependent, dual role of a knowledge hub: to increase the capacity of modelling to meet stakeholder and societal needs under climate change, and to apply that increased capacity to provide new understanding and solutions at the policy and (the focus here) farm scale. While capacity building work across disciplines is time-consuming, difficult, and to a large extent invisible to stakeholders, such work is vital to ensuring that subsequent scientific outcomes reflect best practice, and integrated expertise. Long term, sustained funding of network-based capacity building activities is highlighted as essential to ensuring that the farm-scale modelling work highlighted here can continue to build on ongoing improvements in model quality, flexibility and stakeholder relevance.
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