Abstract: Crop models used as parts of integrated assessments often need to be run on regional, national and global scales. Calibration is an important step in the application procedure, yet on scales like this the process needs to be simplified in order to meet data requirements and computational limits. The question arises if a model calibrated in such a “simple” fashion still performs adequately at field scale, and if parameters not calibrated in the process can nevertheless be used with some confidence in later stages of the assessment. To answer this question, we applied the crop model EPIC to the simulation of sugarcane in Sao Paulo, Brazil. We once calibrated the model using Bayesian calibration to data on yield, aboveground biomass, and root weight measured in four years on two field trials in Sao Paulo. For the second calibration we used a simplified approach and calibrated the model only to FAOSTAT yield data for the whole of Brazil. Both calibrated models were applied to the simulation of stalk yield, aboveground biomass and root weight on a third field trial, and to the simulation of mean yields in Sao Paulo. The results showed that both models were able to adequately depict yields on both scales, but that the model calibrated to only national yield data was not able to accurately simulate root biomass, and to a lesser degree aboveground biomass. We conclude that a simplified calibration performs adequately on both scales, but that non-calibrated parameters may only be used with caution.

Abstract: Ectopic cystatin expression has long been used in plant pest management, but the cysteine protease, targets of these inhibitors, might also have important functions in the control of plant lifespan and stress tolerance that remain poorly characterized. We therefore characterized the effects of expression of the rice cystatin, oryzacystatin-I (OCI), on the growth, development and stress tolerance of crop (soybean) and model (Arabidopsis thaliana) plants. Ectopic OCI expression in soybean enhanced shoot branching and leaf chlorophyll accumulation at later stages of vegetative development and enhanced seed protein contents and decreased the abundance of mRNAs encoding strigolactone synthesis enzymes. The OCI-expressing A. thaliana showed a slow-growth phenotype, with increased leaf numbers and enhanced shoot branching at flowering. The OCI-dependent inhibition of cysteine proteases enhanced drought tolerance in soybean and A. thaliana, photosynthetic CO2 assimilation being much less sensitive to drought-induced inhibition in the OCI-expressing soybean lines. Ectopic OCI expression or treatment with the cysteine protease inhibitor E64 increased lateral root densities in A. thaliana. E64 treatment also increased lateral root densities in the max2-1 mutants that are defective in strigolactone signalling, but not in the max3-9 mutants that are defective in strigolactone synthesis. Taken together, these data provide evidence that OCI-inhibited cysteine proteases participate in the control of growth and stress tolerance through effects on strigolactones. We conclude that cysteine proteases are important targets for manipulation of plant growth, development and stress tolerance, and also seed quality traits.

Abstract: Alongside Robert Costanza and colleagues’ plea to abandon gross domestic product as a measure of national success (see Nature 505, 283–285; 2014), we believe that there is an urgent need to change the way currencies are valued — by using a new ‘carbon standard’ that links economy to ecology. This would work in a similar way to the old gold-exchange standard, except that a country’s currency value would instead be determined by its saved and standing stocks of fossil and non-fossil carbon. Governments would need to decide whether to risk devaluing their currency by depleting carbon stocks — while still honouring a commitment to keep fossil-carbon stocks at 80% as a safeguard against extreme climate change. After the Second World War, huge investments radically altered the economies of the United States, the Soviet Union and the United Kingdom. In the face of climate change, it is now the global energy system that needs reinvention.