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Walkiewicz, A., Bulak, P., Brzezinska, M., Wnuk, E., & Bieganowski, A. (2016). Methane oxidation in heavy metal contaminated Mollic Gleysol under oxic and hypoxic conditions. Environ. Pollut., 213, 403–411.
Abstract: Soils are the largest terrestrial sink for methane (CH4). However, heavy metals may exert toxicity to soil microorganisms, including methanotrophic bacteria. We tested the effect of lead (Pb), zinc (Zn) and nickel (Ni) on CH4 oxidation (1% v/v) and dehydrogenase activity, an index of the activity of the total soil microbial community in Mollic Gleysol soil in oxic and hypoxic conditions (oxia and hypoxia, 20% and 10% v/v O2, respectively). Metals were added in doses corresponding to the amounts permitted of Pb, Zn, Ni in agricultural soils (60, 120, 35 mg kg(-1), respectively), and half and double of these doses. Relatively low metal contents and O2 status reflect the conditions of most agricultural soils of temperate regions. Methane consumption showed high tolerance to heavy metals. The effect of O2 status was stronger than that of metals. CH4 consumption was enhanced under hypoxia, where both the start and the completion of the control and contaminated treatment were faster than under oxic conditions. Dehydrogenase activity, showed higher sensitivity to the contamination (except for low Ni dose), with a stronger effect of heavy metals, than that of the O2 status.
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Van Oosten, M. J., Sharkhuu, A., Batelli, G., Bressan, R. A., & Maggio, A. (2013). The Arabidopsis thaliana mutant air1 implicates SOS3 in the regulation of anthocyanins under salt stress. Plant Mol. Biol., 83(4-5), 405–415.
Abstract: The accumulation of anthocyanins in plants exposed to salt stress has been largely documented. However, the functional link and regulatory components underlying the biosynthesis of these molecules during exposure to stress are largely unknown. In a screen of second site suppressors of the salt overly sensitive3-1 (sos3-1) mutant, we isolated the anthocyanin-impaired-response-1 (air1) mutant. air1 is unable to accumulate anthocyanins under salt stress, a key phenotype of sos3-1 under high NaCl levels (120 mM). The air1 mutant showed a defect in anthocyanin production in response to salt stress but not to other stresses such as high light, low phosphorous, high temperature or drought stress. This specificity indicated that air1 mutation did not affect anthocyanin biosynthesis but rather its regulation in response to salt stress. Analysis of this mutant revealed a T-DNA insertion at the first exon of an Arabidopsis thaliana gene encoding for a basic region-leucine zipper transcription factor. air1 mutants displayed higher survival rates compared to wild-type in oxidative stress conditions, and presented an altered expression of anthocyanin biosynthetic genes such as F3H, F3’H and LDOX in salt stress conditions. The results presented here indicate that AIR1 is involved in the regulation of various steps of the flavonoid and anthocyanin accumulation pathways and is itself regulated by the salt-stress response signalling machinery. The discovery and characterization of AIR1 opens avenues to dissect the connections between abiotic stress and accumulation of antioxidants in the form of flavonoids and anthocyanins.
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van Bussel, L. G. J., Stehfest, E., Siebert, S., Müller, C., & Ewert, F. (2015). Simulation of the phenological development of wheat and maize at the global scale. Glob. Ecol. Biogeogr., 24(9), 1018–1029.
Abstract: AimTo derive location-specific parameters that reflect the geographic differences among cultivars in vernalization requirements, sensitivity to day length (photoperiod) and temperature, which can be used to simulate the phenological development of wheat and maize at the global scale. LocationGlobal. Methods Based on crop calendar observations and literature describing the large-scale patterns of phenological characteristics of cultivars, we developed algorithms to compute location-specific parameters to represent this large-scale pattern. Vernalization requirements were related to the duration and coldness of winter, sensitivity to day length was assumed to be represented by the minimum and maximum day lengths occurring at a location, and sensitivity to temperature was related to temperature conditions during the vegetative development phase of the crop. Results Application of the derived location-specific parameters resulted in high agreement between simulated and observed lengths of the cropping period. Agreement was especially high for wheat, with mean absolute errors of less than 3 weeks. In the main maize cropping regions, cropping periods were over- and underestimated by 0.5-1.5 months. We also found that interannual variability in simulated wheat harvest dates was more realistic when accounting for photoperiod effects. Main conclusions The methodology presented here provides a good basis for modelling the phenological characteristics of cultivars at the global scale. We show that current global patterns of growing season length as described in cropping calendars can be largely reproduced by phenology models if location-specific parameters are derived from temperature and day length indicators. Growing seasons can be modelled more accurately for wheat than for maize, especially in warm regions. Our method for computing parameters for phenology models from temperature and day length offers opportunities to improve the simulation of crop productivity by crop simulation models developed for large spatial areas and for long-term climate impact projections that account for adaptation in the selection of varieties
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Sieber, S., Amjath-Babu, T. S., McIntosh, B. S., Tscherning, K., Müller, K., Helming, K., et al. (2013). Evaluating the characteristics of a non-standardised Model Requirements Analysis (MRA) for the development of policy impact assessment tools. Env. Model. Softw., 49, 53–63.
Abstract: The aim of this paper is to provide a critical analysis of the strengths and weaknesses of a non-standardised Model Requirements Analysis (MRA) used for the purpose of developing the Sustainability Impact Assessment Tool (SIAT). By ‘non-standardised’ we mean not strictly following a published MRA method. The underlying question we are interested in addressing is how non-standardised methods, often employed in research driven projects, compare to defined methods with more standardised structure, with regards their ability to capture model requirements effectively, and with regards their overall usability. Through describing and critically assessing the specific features of the non-standardised MRA employed, the ambition of this paper is to provide insights useful for impact assessment tool (IAT) development. Specifically, the paper will (i) characterise kinds of user requirements relevant to the functionality and design of IATs; (ii) highlight the strengths and weaknesses of non-standardised MRA for user requirements capture, analysis and reflection in the context of IAT; (iii) critically reflect on the process and outcomes of having used a non-standardised MRA in comparison with other more standardised approaches. To accomplish these aims, we first review methods available for IAT development before describing the SIAT development process, including the MRA employed. Major strengths and weaknesses of the MRA method are then discussed in terms of user identification and characterisation, organisational characterisation and embedding, and ability to capture design options for ensuring usability and usefulness. A detailed assessment on the structural differences of MRA with two advanced approaches (Integrated DSS design and goal directed design) and their role in performance of the MRA tool is used to critique the approach employed. The results show that MRA is able to bring thematic integration, establish system performance and technical thresholds as well as detailing quality and transparency guidelines. Nevertheless the discussion points out to a number of deficiencies in application – (i) a need to more effectively characterise potential users, and; (ii) a need to better foster communication among the distinguished roles in the development process. If addressed these deficiencies, SIAT non-standardised MRA could have brought out better outcomes in terms of tool usability and usefulness, and improved embedding of the tool into conditions of targeted end-users. (C) 2013 Elsevier Ltd. All rights reserved.
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Sieber, S., Amjath-Babu, T. S., Jansson, T., Müller, K., Tscherning, K., Graef, F., et al. (2013). Sustainability impact assessment using integrated meta-modelling: Simulating the reduction of direct support under the EU common agricultural policy (CAP). Land Use Policy, 33, 235–245.
Abstract: Assessing the impact of macro-level policy driven land use changes on regional sustainability is an important task that can facilitate complex decision making processes of introducing reforms. The research work demonstrates the ability of Sustainability Impact Assessment Tool (SIAT), a meta-model, in conducting ex ante spatially explicit cross sectoral impact assessments of changes in common agricultural policy (CAP). The meta-model is able to appraise impacts of CAP amendments on land use and their repercussions on multiple indicators of sustainability. The presented study comprehensively analyses the possible impacts of discontinuing direct financial support to farmers under CAP. The simulations of the meta-model are able to reveal the land use changes both at EU and regional levels as well as to bring forth the subsequent changes in a number of indicators representing the regional sustainability (for five case study regions). In a nutshell, the simulations indicate that a reduction in direct support brings in general, a decrease in farmed area, an increase in forested land, less fluctuation in natural vegetation coverage, increase in abandoned arable land area and negligible changes in built-up area despite regionally diverging land use trends. The simulated changes in sustainability indicators for the study regions in consequence to these land use changes show that the discontinuation of subsidies evokes responses that are in general climate friendly (reduction in methane and N2O emissions, diminishing energy use and reduction in global warming potential), economically beneficial (increase in gross value of agriculture) and socially desired (decrease in unemployment rate) as well as environmentally harmful (increase in pesticide use). Even though the appraisals of diversity indicators such as forest deadwood and farmland birds are not conclusive for all regions, the changes are positive for the former indicator and slightly negative for the latter in general. The trade-offs among these regional sustainability indicators using their directional associations are also presented for a comprehensive assessment of the impacts. (C) 2013 Elsevier Ltd. All rights reserved.
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