Records |
Author |
Ben Touhami, H.; Bellocchi, G. |
Title |
Bayesian calibration of the Pasture Simulation model (PaSim) to simulate emissions from long-term grassland sites: a European perspective |
Type |
Conference Article |
Year |
2014 |
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Pages |
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LiveM |
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Conference |
Livestock, Climate Change and Food Security, Madrid, Spain, 2014-05-19 to 2014-05-20 |
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no |
Call Number |
MA @ admin @ |
Serial |
2309 |
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Author |
Quain, M.D.; Makgopa, M.E.; Marquez-Garcia, B.; Comadira, G.; Fernandez-Garcia, N.; Olmos, E.; Schnaubelt, D.; Kunert, K.J.; Foyer, C.H. |
Title |
Ectopic phytocystatin expression leads to enhanced drought stress tolerance in soybean (Glycine max) and Arabidopsis thaliana through effects on strigolactone pathways and can also result in improved seed traits |
Type |
Journal Article |
Year |
2014 |
Publication |
Plant Biotechnology Journal |
Abbreviated Journal |
Plant Biotechnol. J. |
Volume |
12 |
Issue |
7 |
Pages |
903-913 |
Keywords |
Arabidopsis/*genetics/metabolism/physiology; Carbon Dioxide/metabolism; Chlorophyll/metabolism; Cystatins/*genetics/metabolism/physiology; Droughts; Lactones/*metabolism; Oryza/genetics; Phenotype; Plant Proteins/*genetics/metabolism/physiology; Seeds/genetics/metabolism/physiology; Soybeans/*genetics/metabolism/physiology; Stress, Physiological/*genetics; cystatin; cysteine protease; drought tolerance; photosynthesis; seed protein and yield; strigolactone |
Abstract |
Ectopic cystatin expression has long been used in plant pest management, but the cysteine protease, targets of these inhibitors, might also have important functions in the control of plant lifespan and stress tolerance that remain poorly characterized. We therefore characterized the effects of expression of the rice cystatin, oryzacystatin-I (OCI), on the growth, development and stress tolerance of crop (soybean) and model (Arabidopsis thaliana) plants. Ectopic OCI expression in soybean enhanced shoot branching and leaf chlorophyll accumulation at later stages of vegetative development and enhanced seed protein contents and decreased the abundance of mRNAs encoding strigolactone synthesis enzymes. The OCI-expressing A. thaliana showed a slow-growth phenotype, with increased leaf numbers and enhanced shoot branching at flowering. The OCI-dependent inhibition of cysteine proteases enhanced drought tolerance in soybean and A. thaliana, photosynthetic CO2 assimilation being much less sensitive to drought-induced inhibition in the OCI-expressing soybean lines. Ectopic OCI expression or treatment with the cysteine protease inhibitor E64 increased lateral root densities in A. thaliana. E64 treatment also increased lateral root densities in the max2-1 mutants that are defective in strigolactone signalling, but not in the max3-9 mutants that are defective in strigolactone synthesis. Taken together, these data provide evidence that OCI-inhibited cysteine proteases participate in the control of growth and stress tolerance through effects on strigolactones. We conclude that cysteine proteases are important targets for manipulation of plant growth, development and stress tolerance, and also seed quality traits. |
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2016-06-01 |
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English |
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1467-7644 |
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CropM, ft_macsur |
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no |
Call Number |
MA @ admin @ |
Serial |
4740 |
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Author |
Ben Touhami, H.; Lardy, R.; Klumpp, K.; Bellocchi, G. |
Title |
Bayesian calibration of the Pasture Simulation model (PaSim) to simulate emissions from long-term European grassland sites: a case study at Laqueuille (France) |
Type |
Conference Article |
Year |
2013 |
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LiveM |
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Greenhouse Gases & Animal Agriculture 2013, Dublin, Ireland, 2013-06-23 to 2013-06-26 |
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no |
Call Number |
MA @ admin @ |
Serial |
2311 |
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Author |
Bellocchi, G.; Ehrhardt, F. |
Title |
Collaborations with initiatives and projects outside MACSUR and AgMIP – Grassland & Livestock |
Type |
Conference Article |
Year |
2014 |
Publication |
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LiveM |
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International Livestock Modelling and Research Colloquium, Bilbao, Spain, 2014-10-14 to 2014-10-16 |
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no |
Call Number |
MA @ admin @ |
Serial |
2306 |
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Author |
Stefańczyk, E.; Sobkowiak, S.; Brylińska, M.; Śliwka, J. |
Title |
Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland |
Type |
Journal Article |
Year |
2016 |
Publication |
European Journal of Plant Pathology |
Abbreviated Journal |
Eur. J. Plant Pathol. |
Volume |
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Issue |
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Pages |
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Keywords |
ITS; mycotoxin; pathogenicity; Solanum tuberosum; tef-1α; β-tubulin; sequence data; Trichothecenes; identification; fungus; pathogenicity; temperature; sensitivity; zearalenone; strains; disease |
Abstract |
Fusarium spp. belong to the division Ascomycota and cause important plant diseases; these fungi may contaminate food products with mycotoxins, endangering human and animal health. Several Fusarium spp. have been associated with potato dry rot. The most frequent and devastating of these species are F. sambucinum, F. solani and F. oxysporum, depending on the geographic location and the season. Samples of potato tubers with dry rot symptoms were collected, and their putative fungal isolates were identified as Fusarium species using partial nucleotide sequences of the internal transcribed spacer, translation elongation factor 1-α and β-tubulin genes. Among 149 isolates, 12 species were identified. F. oxysporum was the most frequent (45 % of the isolates), followed by F. avenaceum (12.1 %), F. solani (10.7 %) and F. sambucinum (7.4 %). Phylogenetic analyses confirmed the species identifications and revealed a high diversity of F. solani and a low diversity of F. oxysporum. Potential producers of zearalenone and trichothecenes were identified within the obtained isolates using PCR markers. Isolates that were pathogenic to potatoes in laboratory tests were found in four species: F. sambucinum, F. avenaceum, F. culmorum, and F. graminearum. The effects of increased temperature and mixed inoculum on the pathogenicities of chosen species were evaluated. This study adds 434 potato-derived Fusarium sequences to the NCBI GenBank database and demonstrates that the list of Fusarium species and mycotoxins present in potato tubers may be richer than previously believed, regardless of whether these species cause dry rot or live as saprophytes. |
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English |
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0929-1873 1573-8469 |
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CropM, ft_macsur |
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no |
Call Number |
MA @ admin @ |
Serial |
4721 |
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