Abstract: Agricultural nitrous oxide (N2O) pollution resulting from the use of synthetic fertilizers represents a significant contribution to anthropogenic greenhouse gas emissions, providing a rationale for reduced use of nitrogen (N) fertilizers. Nitrogen limitation results in extensive systems rebalancing that remodels metabolism and defence processes. To analyse the regulation underpinning these responses, barley (Horedeum vulgare) seedlings were grown for 7 d under N-deficient conditions until net photosynthesis was 50% lower than in N-replete controls. Although shoot growth was decreased there was no evidence for the induction of oxidative stress despite lower total concentrations of N-containing antioxidants. Nitrogen-deficient barley leaves were rich in amino acids, sugars and tricarboxylic acid cycle intermediates. In contrast to N-replete leaves one-day-old nymphs of the green peach aphid (Myzus persicae) failed to reach adulthood when transferred to N-deficient barley leaves. Transcripts encoding cell, sugar and nutrient signalling, protein degradation and secondary metabolism were over-represented in N-deficient leaves while those associated with hormone metabolism were similar under both nutrient regimes with the exception of mRNAs encoding proteins involved in auxin metabolism and responses. Significant similarities were observed between the N-limited barley leaf transcriptome and that of aphid-infested Arabidopsis leaves. These findings not only highlight significant similarities between biotic and abiotic stress signalling cascades but also identify potential targets for increasing aphid resistance with implications for the development of sustainable agriculture.

Abstract: MACSUR — Modelling European Agriculture with Climate Change for Food Security — is a  knowledge hub that was formally created in June 2012 as a European scientific network.  The strategic aim of the knowledge hub is to create a coordinated and globally visible  network of European researchers and research groups, with intra- and interdisciplinary  interaction and shared expertise creating synergies for the development of scientific  resources (data, models, methods) to model the impacts of climate change on agriculture  and related issues. This objective encompasses a wide range of political and sociological  aspects, as well as the technical development of modelling capacity through impact  assessments at different scales and assessing uncertainties in model outcomes. We achieve  this through model intercomparisons and model improvements, harmonization and  exchange of data sets, training in the selection and use of models, assessment of benefits  of ensemble modelling, and cross-disciplinary linkages of models and tools. The project  engages with a diverse range of stakeholder groups and to support the development of  resources for capacity building of individuals and countries. Commensurate with this broad  challenge, a network of currently 300 scientists (measured by the number of individuals on  the central e-mail list) from 18 countries evolved from the original set of research groups  selected by FACCE.   In the spirit of creating and maintaining a network for intra- and interdisciplinary  knowledge exchange, network activities focused on meetings of researchers for sharing  expertise and, depending on group resources (both financial and personnel), development  of collaborative research activities. The outcome of these activities is the enhanced  knowledge of the individual researchers within the network, contributions to conference  presentations and scholarly papers, input to stakeholders and the general public, organised  courses for students, junior and senior scientists. The most visible outcome are the  scientific results of the network activities, represented in the contributions of MACSUR  members to the impressive number of more than 200 collaborative papers in peer-reviewed  publications.   Here, we present a selection of overview and cross-disciplinary papers which include  contributions from MACSUR members. It highlights the major scientific challenges  addressed, and the methodological solutions and insights obtained. Over and above these  highlights, major achievements have been reached regarding data collection, data  processing, evaluation, model testing, modelling assessments of the effects of agriculture  on ecosystem services, policy, and development of scenarios. Details on these  achievements in the context of MACSUR can be found in our online publication FACCE  MACSUR Reports at http://ojs.macsur.eu.