Brylińska, M., Sobkowiak, S., Stefańczyk, E., & Śliwka, J. (2016). Potato cultivation system affects population structure of Phytophthora infestans. Fungal Ecology, 20, 132–143. Abstract: Phytophthora infestans is one of the most destructive potato pathogens. Many factors influence the population structure of P. infestans, including migration, climate and type of potato cultivation. Here, we analyse 365 P. infestans isolates collected from three regions of Poland over three years. We determined mating type, mitochondrial haplotype, resistance to metalaxyl, virulence and polymorphism at 14 simple sequence repeat (SSR) loci. Analysis of SSR markers showed high genetic diversity associated with this population. Model-based structure analysis grouped 299 unique genotypes into four main clusters. The P. infestans isolates collected from the Mlochow region, which has the most intensive level of potato cultivation, formed a distinct cluster, indicating a strong effect of the cultivation system on pathogen population structure. Three clusters contained isolates with frequent presence of three alleles at one locus, which may affect their capacity for sexual reproduction and preserve groups of fit genotypes that propagate asexually. (C) 2016 Elsevier Ltd and The British Mycological Society. Keywords: SSR; Population genetic structure; Late blight; Potato; late blight resistance; mating-type; microsatellite markers; phenotypic diversity; sexual reproduction; genotypic diversity; nordic countries; severe outbreaks; sarpo mira; pathogenicity Permanent link \| Save citation: RTF PDF LaTeX Markdown \| Export record: BibTeX Endnote ISI RIS Atom XML MODS XML ODF XML Word XML

Abstract: Phytophthora infestans is one of the most destructive potato pathogens. Many factors influence the population structure of P. infestans, including migration, climate and type of potato cultivation. Here, we analyse 365 P. infestans isolates collected from three regions of Poland over three years. We determined mating type, mitochondrial haplotype, resistance to metalaxyl, virulence and polymorphism at 14 simple sequence repeat (SSR) loci. Analysis of SSR markers showed high genetic diversity associated with this population. Model-based structure analysis grouped 299 unique genotypes into four main clusters. The P. infestans isolates collected from the Mlochow region, which has the most intensive level of potato cultivation, formed a distinct cluster, indicating a strong effect of the cultivation system on pathogen population structure. Three clusters contained isolates with frequent presence of three alleles at one locus, which may affect their capacity for sexual reproduction and preserve groups of fit genotypes that propagate asexually. (C) 2016 Elsevier Ltd and The British Mycological Society.

Keywords: SSR; Population genetic structure; Late blight; Potato; late blight resistance; mating-type; microsatellite markers; phenotypic diversity; sexual reproduction; genotypic diversity; nordic countries; severe outbreaks; sarpo mira; pathogenicity

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