Abstract: The assessment of net ecosystem exchange and respiration of ecosystem of terrestrial ecosystems is necessary to improve our knowledge about carbon cycle in nature. Here we present measurements of CO2 fluxes for a winter wheat temperate climate ecosystem (buckwheat in the previous years) located in the Lubelskie province (eastern Poland) using a closed dynamic chamber system over a 2013 vegetation season. Measurements of carbon dioxide emission from soils and its assimilation by plants were carried out on a typical for Lubelskie highland arable land located in the Stany Nowe (N50o49’17.0555”, E22o16’28.51”, height 243m above sea level) using the set of two chambers (transparent and dark). Carbon dioxide fluxes have been measured by EGM-4 PP Systems sensor during fixed stages of the plant growing season. During the experiment carbon emission from soil ranged from 151 to 764 mg C·m-2·h-1 and its assimilation by plants ranged from -148 (emission) to 1585 mg C·m-2·h-1. We found substantial differences in emission and assimilation of carbon in the winter wheat ecosystem. This, along with other measurements (meteorological factors and soil and plant parameters) carried out in the Stany Nowe can be used as a high quality data to verify various models of  emission of greenhouse gases. The chamber technique occurs to be  a useful tool for determining carbon dioxide exchange between ecosystem surface and the atmosphere.